TY - JOUR
T1 - Development and characterization of a hybridoma-derived antibody (Aby 1A1) with specificity to canine thymocytes and peripheral T lymphocytes.
AU - Krawiec, D. R.
AU - Muscoplat, C. C.
PY - 1984/3
Y1 - 1984/3
N2 - A hybridoma (1A1)-producing antibody with reactivity to canine T-lymphocyte surface antigens was produced, using standard fusion techniques. Antibody secreted from this hybridoma was tested for specific anti-canine lymphocyte activity, using an enzyme-linked immunosorbent assay, an indirect-fluorescent antibody assay, and cytotoxicity assay. Antibody 1A1 reacted with canine T lymphocytes, but not with B lymphocytes, and with brain tissue and connective tissue. The antibody was also characterized. Its heavy chain had a molecular weight (mol wt) of 80,000 and its light chain was 28,000 mol wt. The Aby 1A1 is a monoclonal immunoglobulin M with a kappa light chain, and it binds poorly to Staphylococcus A.
AB - A hybridoma (1A1)-producing antibody with reactivity to canine T-lymphocyte surface antigens was produced, using standard fusion techniques. Antibody secreted from this hybridoma was tested for specific anti-canine lymphocyte activity, using an enzyme-linked immunosorbent assay, an indirect-fluorescent antibody assay, and cytotoxicity assay. Antibody 1A1 reacted with canine T lymphocytes, but not with B lymphocytes, and with brain tissue and connective tissue. The antibody was also characterized. Its heavy chain had a molecular weight (mol wt) of 80,000 and its light chain was 28,000 mol wt. The Aby 1A1 is a monoclonal immunoglobulin M with a kappa light chain, and it binds poorly to Staphylococcus A.
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M3 - Article
C2 - 6370057
AN - SCOPUS:0021396439
SN - 0002-9645
VL - 45
SP - 491
EP - 498
JO - American journal of veterinary research
JF - American journal of veterinary research
IS - 3
ER -