Differential induction of type I and type III interferons by swine and human origin H1N1 influenza a viruses in porcine airway epithelial cells

Venkatramana D. Krishna, Erin Roach, Nathan A. Zaidman, Angela Panoskaltsis-Mortari, Jessica H. Rotschafer, Scott M. O'Grady, Maxim C J Cheeran

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Interferons (IFNs) have been shown to inhibit influenza A virus (IAV) replication and play an essential role in controlling viral infection.Here we studied the kinetics and magnitude of induction of type I and type III IFN transcripts by primary porcine airway epithelial cells (pAECs) in response to swine and human origin IAV.We observed that swine influenza viruses (SIV) replicate more efficiently than the human pandemic influenza A/California/ 2009 (pH1N1 CA/09) in pAECs.Interestingly, we also found significant difference in kinetics of IFN-β,IFN-eλ1 and IFN-eλ3 gene expression by these viruses.While there was delay of up to 12 hours post infection (h p.i.) in induction of IFN genes in pAECs infected with swine IAV A/Sw/Illinois/2008 (H1N1 IL/08), human pH1N1 CA/09 rapidly induced IFN-β,IFN-eλ1 and IFN-eλ3 gene expression as early as 4 h p.i. However,the magnitude of IFN-β and IFN-eλ3 induction at 24 h p.i. was not significantly different between the viral strains tested. Additionally, we found that swine H1N1 IL/08 was less sensitive to dsRNA induced antiviral response compared to human pH1N1 CA/09. Our data suggest that the human and swine IAVs differ in their ability to induce and respond to type I and type III interferons in swine cells. Swine origin IAV may have adapted to the pig host by subverting innate antiviral responses to viral infection.

Original languageEnglish (US)
Article numbere0138704
JournalPloS one
Volume10
Issue number9
DOIs
StatePublished - Sep 18 2015

Bibliographical note

Publisher Copyright:
© 2015 Krishna et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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