Differential neuronal and glial behavior on flat and micro patterned chitosan films

Marta Mattotti; Zaida Alvarez; Luis Delgado; Miguel A. Mateos-Timoneda; Conrado Aparicio; Josep A. Planell; Soledad Alcántara; Elisabeth Engel

doi:10.1016/j.colsurfb.2017.07.045

Differential neuronal and glial behavior on flat and micro patterned chitosan films

Marta Mattotti, Zaida Alvarez, Luis Delgado, Miguel A. Mateos-Timoneda, Conrado Aparicio, Josep A. Planell, Soledad Alcántara, Elisabeth Engel

Biomaterials

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Chitosan is a biodegradable natural polysaccharide that has been widely studied for regenerative purposes in the central nervous system. In this study we assessed the in vitro glial and neuronal cells response to chitosan either flat or patterned with grooves in the micrometric range. Chitosan demonstrated to be a good substrate for the attachment and growth of both neurons and glial cells. Chitosan micropatterns promoted glial cell maturation, suggesting astroglial activation. Nevertheless, those mature/reactive glial cells were permissive for axonal growth. Axons aligned and organized along the patterned grooves and the size of the linear topographic patterns is also affecting neurite and cell response. Patterns with 10 μm width induced fasciculation of axons, which can be useful for CNS tissue engineering substrates when precise orientation of the axonal outgrowth is desired.

Original language	English (US)
Pages (from-to)	569-577
Number of pages	9
Journal	Colloids and Surfaces B: Biointerfaces
Volume	158
DOIs	https://doi.org/10.1016/j.colsurfb.2017.07.045
State	Published - Oct 1 2017

Bibliographical note

Funding Information:
This study was supported in part by grants from Spain's Ministerio de Ciencia e Innovación [MAT2011-29778-C02-01] and [MAT2011-29778-C02-02] co-financed by the European Regional Development Fund, to J.A.P. and S.A respectively, from 2014 Generalitat de Catalunya SGR 1178 to S.A., as well as through the fellowship FPU AP2008-01868 to M.M.

Publisher Copyright:
© 2017 Elsevier B.V.

Keywords

Brain
Chitosan
Glia
Micropattern
Neuron

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abstract = "Chitosan is a biodegradable natural polysaccharide that has been widely studied for regenerative purposes in the central nervous system. In this study we assessed the in vitro glial and neuronal cells response to chitosan either flat or patterned with grooves in the micrometric range. Chitosan demonstrated to be a good substrate for the attachment and growth of both neurons and glial cells. Chitosan micropatterns promoted glial cell maturation, suggesting astroglial activation. Nevertheless, those mature/reactive glial cells were permissive for axonal growth. Axons aligned and organized along the patterned grooves and the size of the linear topographic patterns is also affecting neurite and cell response. Patterns with 10 μm width induced fasciculation of axons, which can be useful for CNS tissue engineering substrates when precise orientation of the axonal outgrowth is desired.",

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AU - Planell, Josep A.

AU - Alcántara, Soledad

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Differential neuronal and glial behavior on flat and micro patterned chitosan films

Abstract

Bibliographical note

Keywords

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