TY - JOUR
T1 - Direct immobilization of manganese chelates on silica nanospheres for MRI applications
AU - Pálmai, Marcell
AU - Pethő, Adrienn
AU - Nagy, Lívia Naszályi
AU - Klébert, Szilvia
AU - May, Zoltán
AU - Mihály, Judith
AU - Wacha, András
AU - Jemnitz, Katalin
AU - Veres, Zsuzsanna
AU - Horváth, Ildikó
AU - Szigeti, Krisztián
AU - Máthé, Domokos
AU - Varga, Zoltán
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/7/15
Y1 - 2017/7/15
N2 - The development of tissue specific magnetic resonance imaging (MRI) contrast agents (CAs) is very desirable to achieve high contrast ratio combined with excellent anatomical details. To this end, we introduce a highly effective manganese(II) containing silica material, with the aim to shorten the longitudinal (T 1 ) relaxation time. The microporous silica nanospheres (MSNSs) with enlarged porosity and specific surface area were prepared by a surfactant assisted aqueous method. Subsequently, the surface silanol groups were amino-functionalized, reacted with diethylenetriaminepentaacetic (DTPA) dianhydride and finally deposited with Mn 2+ . After comprehensive characterization, the MRI properties of functionalized MSNSs were investigated. The resulting nanospheres demonstrated substantial contrast enhancement during the in vitro MRI investigations, which was also evidenced by significant contrast enhancement on T 1 -weighted MR images in vivo. Moreover, in vitro cytotoxicity assay of functionalized MSNSs on hepatocyte mono- and hepatocyte-Kuppfer cell co-cultures showed no significant decrease in cell viability. Our findings confirmed our hypothesis, that Mn 2+ -chelating MSNSs are appropriate candidates for liver-specific T 1 -weighted MRI CAs with high relaxivities (r 1 = 7.18 mM −1 s −1 ).
AB - The development of tissue specific magnetic resonance imaging (MRI) contrast agents (CAs) is very desirable to achieve high contrast ratio combined with excellent anatomical details. To this end, we introduce a highly effective manganese(II) containing silica material, with the aim to shorten the longitudinal (T 1 ) relaxation time. The microporous silica nanospheres (MSNSs) with enlarged porosity and specific surface area were prepared by a surfactant assisted aqueous method. Subsequently, the surface silanol groups were amino-functionalized, reacted with diethylenetriaminepentaacetic (DTPA) dianhydride and finally deposited with Mn 2+ . After comprehensive characterization, the MRI properties of functionalized MSNSs were investigated. The resulting nanospheres demonstrated substantial contrast enhancement during the in vitro MRI investigations, which was also evidenced by significant contrast enhancement on T 1 -weighted MR images in vivo. Moreover, in vitro cytotoxicity assay of functionalized MSNSs on hepatocyte mono- and hepatocyte-Kuppfer cell co-cultures showed no significant decrease in cell viability. Our findings confirmed our hypothesis, that Mn 2+ -chelating MSNSs are appropriate candidates for liver-specific T 1 -weighted MRI CAs with high relaxivities (r 1 = 7.18 mM −1 s −1 ).
KW - DTPA
KW - Manganese(II) chelate
KW - Nanoparticulate MRI contrast agent
KW - Silica nanospheres
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U2 - 10.1016/j.jcis.2017.03.053
DO - 10.1016/j.jcis.2017.03.053
M3 - Article
C2 - 28343127
AN - SCOPUS:85016079481
SN - 0021-9797
VL - 498
SP - 298
EP - 305
JO - Journal of Colloid And Interface Science
JF - Journal of Colloid And Interface Science
ER -