Dissecting meiotic recombination based on tetrad analysis by single-microspore sequencing in maize

Xiang Li, Lin Li, Jianbing Yan

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52 Scopus citations

Abstract

Meiotic recombination drives eukaryotic sexual reproduction and the generation of genome diversity. Tetrad analysis, which examines the four chromatids resulting from a single meiosis, is an ideal method to study the mechanisms of homologous recombination. Here we develop a method to isolate the four microspores from a single tetrad in maize for the purpose of whole-genome sequencing. A high-resolution recombination map reveals that crossovers are unevenly distributed across the genome and are more likely to occur in the genic than intergenic regions, especially common in the 5′- and 3′-end regions of annotated genes. The direct detection of genomic exchanges suggests that conversions likely occur in most crossover tracts. Negative crossover interference and weak chromatid interference are observed at the population level. Overall, our findings further our understanding of meiotic recombination with implications for both basic and applied research.

Original languageEnglish (US)
Article number6648
JournalNature communications
Volume6
DOIs
StatePublished - Mar 2015

Bibliographical note

Funding Information:
We appreciated the helpful comments on the manuscript from Dr Marilyn Warburton. This research was supported by the National Natural Science Foundation of China (31123009 and 31222041) and the National Hi-Tech Research and Development Program of China (2012AA10A307) and the National Youth Top-notch Talent Support Program.

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