DNA electrophoresis in a sparse ordered post array

Jia Ou, Jaeseol Cho, Daniel W. Olson, Kevin D. Dorfman

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

We present a study of the electrophoresis of long DNA in a strong electric field through a hexagonal array of cylindrical microscale posts spaced such that the pore size is commensurate with equilibrium coil size of the DNA. Experimental mobility, dispersivity, and videomicroscopy data indicate that the DNA frequently collide with the posts, contradicting previous Brownian dynamics studies using a uniform electric field. We demonstrate via simulations that the frequent collisions, which are essential to separations in these devices, are due to the nonuniform electric field, highlighting the importance of accounting for electric-field gradients when modeling DNA transport in microfluidic devices.

Original languageEnglish (US)
Article number061904
JournalPhysical Review E - Statistical, Nonlinear, and Soft Matter Physics
Volume79
Issue number6
DOIs
StatePublished - Jun 4 2009

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