TY - JOUR
T1 - DNA electrophoresis in a sparse ordered post array
AU - Ou, Jia
AU - Cho, Jaeseol
AU - Olson, Daniel W.
AU - Dorfman, Kevin D.
PY - 2009/6/4
Y1 - 2009/6/4
N2 - We present a study of the electrophoresis of long DNA in a strong electric field through a hexagonal array of cylindrical microscale posts spaced such that the pore size is commensurate with equilibrium coil size of the DNA. Experimental mobility, dispersivity, and videomicroscopy data indicate that the DNA frequently collide with the posts, contradicting previous Brownian dynamics studies using a uniform electric field. We demonstrate via simulations that the frequent collisions, which are essential to separations in these devices, are due to the nonuniform electric field, highlighting the importance of accounting for electric-field gradients when modeling DNA transport in microfluidic devices.
AB - We present a study of the electrophoresis of long DNA in a strong electric field through a hexagonal array of cylindrical microscale posts spaced such that the pore size is commensurate with equilibrium coil size of the DNA. Experimental mobility, dispersivity, and videomicroscopy data indicate that the DNA frequently collide with the posts, contradicting previous Brownian dynamics studies using a uniform electric field. We demonstrate via simulations that the frequent collisions, which are essential to separations in these devices, are due to the nonuniform electric field, highlighting the importance of accounting for electric-field gradients when modeling DNA transport in microfluidic devices.
UR - http://www.scopus.com/inward/record.url?scp=67650886441&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67650886441&partnerID=8YFLogxK
U2 - 10.1103/PhysRevE.79.061904
DO - 10.1103/PhysRevE.79.061904
M3 - Article
C2 - 19658521
AN - SCOPUS:67650886441
SN - 1539-3755
VL - 79
JO - Physical Review E - Statistical, Nonlinear, and Soft Matter Physics
JF - Physical Review E - Statistical, Nonlinear, and Soft Matter Physics
IS - 6
M1 - 061904
ER -