DNA methylation in a sea lamprey vasotocin receptor gene promoter correlates with tissue- and life-stage-specific mRNA expression

Sally A. Mayasich; Lynne T Bemis; Benjamin L Clarke

doi:10.1016/j.cbpb.2016.07.007

DNA methylation in a sea lamprey vasotocin receptor gene promoter correlates with tissue- and life-stage-specific mRNA expression

Sally A. Mayasich, Lynne T Bemis, Benjamin L Clarke

Biomedical Sciences

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4 Scopus citations

Abstract

The jawless vertebrate sea lamprey (Petromyzon marinus) genome has a different structure from both invertebrates and jawed vertebrates featuring high guanine–cytosine (GC) content. This raises the question of whether DNA methylation of cytosine–guanine (CpG) dinucleotides could function to regulate lamprey gene transcription. We previously characterized a lamprey arginine vasotocin (AVT) receptor gene (Pm807) possessing characteristics of both arginine vasopressin (AVP) V1A and oxytocin (OXT) receptor genes of jawed vertebrates. Lamprey Pm807 mRNA is highly expressed in adult heart and larval liver but not expressed in adult liver. Using high-resolution melt (HRM) PCR on bisulfite-converted DNA, we pinpointed a region with tissue-specific differences in DNA melt characteristics, indicating differences in methylation level. Sequencing revealed a pattern of methylation at specific CpGs at consistently higher levels in adult heart and larval liver than adult liver. These CpGs are associated with putative transcription factor binding sequences organized similarly to functional OXTR promoters in mammals, suggesting functional similarity in lamprey gene transcription regulation.

Original language	English (US)
Pages (from-to)	56-66
Number of pages	11
Journal	Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume	202
DOIs	https://doi.org/10.1016/j.cbpb.2016.07.007
State	Published - Dec 1 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Inc.

Keywords

DNA methylation
Estrogen response element
Oxytocin receptor
Sea lamprey
Vasotocin receptor
Zinc-finger transcription factors

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DNA methylation in a sea lamprey vasotocin receptor gene promoter correlates with tissue- and life-stage-specific mRNA expression. / Mayasich, Sally A.; Bemis, Lynne T ; Clarke, Benjamin L.
In: Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology, Vol. 202, 01.12.2016, p. 56-66.

Research output: Contribution to journal › Article › peer-review

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abstract = "The jawless vertebrate sea lamprey (Petromyzon marinus) genome has a different structure from both invertebrates and jawed vertebrates featuring high guanine–cytosine (GC) content. This raises the question of whether DNA methylation of cytosine–guanine (CpG) dinucleotides could function to regulate lamprey gene transcription. We previously characterized a lamprey arginine vasotocin (AVT) receptor gene (Pm807) possessing characteristics of both arginine vasopressin (AVP) V1A and oxytocin (OXT) receptor genes of jawed vertebrates. Lamprey Pm807 mRNA is highly expressed in adult heart and larval liver but not expressed in adult liver. Using high-resolution melt (HRM) PCR on bisulfite-converted DNA, we pinpointed a region with tissue-specific differences in DNA melt characteristics, indicating differences in methylation level. Sequencing revealed a pattern of methylation at specific CpGs at consistently higher levels in adult heart and larval liver than adult liver. These CpGs are associated with putative transcription factor binding sequences organized similarly to functional OXTR promoters in mammals, suggesting functional similarity in lamprey gene transcription regulation.",

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DNA methylation in a sea lamprey vasotocin receptor gene promoter correlates with tissue- and life-stage-specific mRNA expression

Abstract

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