Dopamine receptors influence vasoactive intestinal peptide release from turkey hypothalamic explants

Yupaporn Chaiseh, Orlan M. Youngren, M E Elhalawani

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Vasoactive intestinal peptide (VIP) is a significant prolactin-releasing factor (PRF) in avian species, and dopamine (DA) exhibits both a stimulatory and inhibitory influence upon this prolactin (PRL) secretion. The stimulatory effect of DA upon PRL release appears to be mediated by VIP. This study investigated DAergic actions upon VIP release using turkey hypothalamic explants perifused with DA and its agonists or antagonists. VIP release was stimulated by DA in a dose-dependent manner (10 nmol DA/min, from 67.2 ± 3.9 to 164.3 ± 3.1 pg/5 min; 100 nmol DA/min, from 70.1 ± 3.2 to 291.0 ± 7.5 pg/5 min; 1,000 nmol DA/min, from 72.0 ± 4.8 to 501.0 ± 24.7 pg/5 min). The D1 DA receptor antagonist (R+)-SCH-23390 HC1 completely negated the stimulatory effect of DA (100 nmol/min) upon VIP release. Perifusion with the D2 DA receptor antagonist S(-)-eticlopride HC1 by itself stimulated VIP release from the hypothalamic explants, increasing VIP from 38.1 ± 5.3 to 161.9 ± 9.7 pg/5 min, where release stabilized until perifusion was terminated. The D1 DA agonist (+)-SKF-38393 HC1 increased VIP release from 52.7 ± 4.6 to 192.6 ± 16.9 pg/5 min, and this stimulated release was partially inhibited by the D2 DA receptor agonist R(-)-NPA HC1 (from 192.6 ± 16.9 to 139.7 ± 13.8 pg/5 min). These results suggest that VIP secretion is in part regulated by possible opposite actions between stimulatory D1 and inhibitory D2 DA receptors in the turkey hypothalamus.

Original languageEnglish (US)
Pages (from-to)423-429
Number of pages7
JournalNeuroendocrinology
Volume65
Issue number6
DOIs
StatePublished - Jan 1 1997

Keywords

  • Birds
  • Catecholamine receptor
  • Catecholamines
  • Vasoactive intestinal peptide

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