TY - JOUR
T1 - Draft genome of omphalotus olearius provides a predictive framework for sesquiterpenoid natural product biosynthesis in basidiomycota
AU - Wawrzyn, Grayson T.
AU - Quin, Maureen B.
AU - Choudhary, Swati
AU - López-Gallego, Fernando
AU - Schmidt-Dannert, Claudia
N1 - Funding Information:
This research was supported by the National Institutes of Health Grant GM080299 (to C.S-D.). G.T.W. was supported by a predoctoral National Institutes of Health traineeship (GM08700).
PY - 2012/6/22
Y1 - 2012/6/22
N2 - The secondary metabolome of Basidiomycota represents a largely uncharacterized source of pharmaceutically relevant natural products. Terpenoids are the primary class of bioactive compounds isolated from mushrooms. The Jack O'Lantern mushroom Omphalotus olearius was identified 50 years ago as a prolific producer of anticancer illudin sesquiterpenoids; however, to date there have been exceptionally few studies into the biosynthesis of these important compounds. Here, we report the draft genome sequence of O. olearius, which reveals a diverse network of sesquiterpene synthases and two metabolic gene clusters associated with illudin biosynthesis. Characterization of the sesquiterpene synthases enabled a comprehensive survey of all currently available Basidiomycota genomes, thereby creating a predictive resource for terpenoid natural product biosynthesis in these organisms. Our results will facilitate discovery and biosynthetic production of unique pharmaceutically relevant bioactive compounds from Basidiomycota.
AB - The secondary metabolome of Basidiomycota represents a largely uncharacterized source of pharmaceutically relevant natural products. Terpenoids are the primary class of bioactive compounds isolated from mushrooms. The Jack O'Lantern mushroom Omphalotus olearius was identified 50 years ago as a prolific producer of anticancer illudin sesquiterpenoids; however, to date there have been exceptionally few studies into the biosynthesis of these important compounds. Here, we report the draft genome sequence of O. olearius, which reveals a diverse network of sesquiterpene synthases and two metabolic gene clusters associated with illudin biosynthesis. Characterization of the sesquiterpene synthases enabled a comprehensive survey of all currently available Basidiomycota genomes, thereby creating a predictive resource for terpenoid natural product biosynthesis in these organisms. Our results will facilitate discovery and biosynthetic production of unique pharmaceutically relevant bioactive compounds from Basidiomycota.
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U2 - 10.1016/j.chembiol.2012.05.012
DO - 10.1016/j.chembiol.2012.05.012
M3 - Article
C2 - 22726691
AN - SCOPUS:84862732885
SN - 1074-5521
VL - 19
SP - 772
EP - 783
JO - Chemistry and Biology
JF - Chemistry and Biology
IS - 6
ER -
