TY - JOUR
T1 - Effect of prostaglandin, leukotriene, and arachidonic acid on experimental otitis media with effusion in chinchillas.
AU - Jung, T. T.
AU - Park, Y. M.
AU - Schlund, D.
AU - Weeks, D.
AU - Miller, S.
AU - Wong, O.
AU - Juhn, S. K.
PY - 1990/6
Y1 - 1990/6
N2 - Although previous studies have shown that prostaglandins (PGs), leukotrienes (LTs), and other arachidonic acid (AA) metabolites play an important role in the pathogenesis of otitis media with effusion (OME), the exact role of each AA metabolite in OME is still unknown. The purpose of this study was to examine the effect of several individual AA metabolites alone or in combination and AA itself on experimental otitis media in chinchillas. Normal chinchillas were inoculated daily with normal saline, PGE2, LTC4, LTC4 + PGE2, and AA through the superior bullae over 7 days. Animals were followed by otoscopy and tympanometry, samples of middle ear effusion were collected for biochemical assay, and temporal bones were processed for histopathology. The highest number of ears that developed OME was in the group inoculated with PGE2 + LTC4. The degree of inflammatory change was more pronounced in groups injected with LTC4 than any other group. The findings of this study suggest that eicosanoids PGE2, LTC4, and AA alone or in combination inoculated into the middle ear can induce OME.
AB - Although previous studies have shown that prostaglandins (PGs), leukotrienes (LTs), and other arachidonic acid (AA) metabolites play an important role in the pathogenesis of otitis media with effusion (OME), the exact role of each AA metabolite in OME is still unknown. The purpose of this study was to examine the effect of several individual AA metabolites alone or in combination and AA itself on experimental otitis media in chinchillas. Normal chinchillas were inoculated daily with normal saline, PGE2, LTC4, LTC4 + PGE2, and AA through the superior bullae over 7 days. Animals were followed by otoscopy and tympanometry, samples of middle ear effusion were collected for biochemical assay, and temporal bones were processed for histopathology. The highest number of ears that developed OME was in the group inoculated with PGE2 + LTC4. The degree of inflammatory change was more pronounced in groups injected with LTC4 than any other group. The findings of this study suggest that eicosanoids PGE2, LTC4, and AA alone or in combination inoculated into the middle ear can induce OME.
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U2 - 10.1177/00034894900990s608
DO - 10.1177/00034894900990s608
M3 - Article
C2 - 2161636
AN - SCOPUS:0025357995
SN - 0096-8056
VL - 148
SP - 28
EP - 32
JO - The Annals of otology, rhinology & laryngology. Supplement
JF - The Annals of otology, rhinology & laryngology. Supplement
IS - 6 II SUPPL. 148
ER -