Effect of tri-o-cresyl phosphate and methamidophos on 45Ca uptake by brain synaptosomes in hens

Yi Jun Wu; Ping An Chang; Ming Li; Yu Xia Li; Wei Li

doi:10.1016/S0048-3575(03)00082-8

Effect of tri-o-cresyl phosphate and methamidophos on ⁴⁵Ca uptake by brain synaptosomes in hens

Yi Jun Wu, Ping An Chang, Ming Li, Yu Xia Li, Wei Li

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The effect of tri-o-cresyl phosphate (TOCP) and methamidophos (MET) on potassium-stimulated ⁴⁵Ca uptake by brain synaptosomes in hens was studied. An in vivo test showed that TOCP increased potassium-stimulated calcium uptake 2 h after its administration, but that verapamil suppressed the enhancement of this calcium uptake. An in vitro test showed that lower concentrations of TOCP stimulated calcium uptake by synaptosomes, but that higher concentrations inhibited the uptake. In contrast, all tested concentrations of MET obviously inhibited calcium uptake; however, since calcium uptake was decreased by the administration of verapamil plus either TOCP or MET, the mechanism by which TOCP affects the voltage-operated calcium channel may be different from that of MET. The disruption of calcium homeostasis may be involved in organophosphate-induced delayed neurotoxicity (OPIDN). Calcium channel blocker may ameliorate OPIDN by maintaining calcium homeostasis in nerve cells.

Original language	English (US)
Pages (from-to)	18-23
Number of pages	6
Journal	Pesticide Biochemistry and Physiology
Volume	77
Issue number	1
DOIs	https://doi.org/10.1016/S0048-3575(03)00082-8
State	Published - Sep 2003
Externally published	Yes

Bibliographical note

Funding Information:
This project was supported by grants from the National Nature Science Foundation of China (No. 39970151), CAS Innovation Program (KSCX1-SW-13-02-04; KSCX3-IOZ-04) and the CAS Life Sciences Special Fund (supported by the Ministry of Finance) (STZ98-2-13). Authors thank Dr. Carey Pope for helpful comments on earlier draft, and Dr. Ron Moorhouse for polishing the English.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

Calcium uptake
Methamidophos (MET)
Organophosphate-induced delayed neurotoxicity (OPIDN)
Tri-o-cresyl phosphate (TOCP)
Verapamil

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title = "Effect of tri-o-cresyl phosphate and methamidophos on 45Ca uptake by brain synaptosomes in hens",

abstract = "The effect of tri-o-cresyl phosphate (TOCP) and methamidophos (MET) on potassium-stimulated 45Ca uptake by brain synaptosomes in hens was studied. An in vivo test showed that TOCP increased potassium-stimulated calcium uptake 2 h after its administration, but that verapamil suppressed the enhancement of this calcium uptake. An in vitro test showed that lower concentrations of TOCP stimulated calcium uptake by synaptosomes, but that higher concentrations inhibited the uptake. In contrast, all tested concentrations of MET obviously inhibited calcium uptake; however, since calcium uptake was decreased by the administration of verapamil plus either TOCP or MET, the mechanism by which TOCP affects the voltage-operated calcium channel may be different from that of MET. The disruption of calcium homeostasis may be involved in organophosphate-induced delayed neurotoxicity (OPIDN). Calcium channel blocker may ameliorate OPIDN by maintaining calcium homeostasis in nerve cells.",

keywords = "Calcium uptake, Methamidophos (MET), Organophosphate-induced delayed neurotoxicity (OPIDN), Tri-o-cresyl phosphate (TOCP), Verapamil",

author = "Wu, {Yi Jun} and Chang, {Ping An} and Ming Li and Li, {Yu Xia} and Wei Li",

note = "Funding Information: This project was supported by grants from the National Nature Science Foundation of China (No. 39970151), CAS Innovation Program (KSCX1-SW-13-02-04; KSCX3-IOZ-04) and the CAS Life Sciences Special Fund (supported by the Ministry of Finance) (STZ98-2-13). Authors thank Dr. Carey Pope for helpful comments on earlier draft, and Dr. Ron Moorhouse for polishing the English. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

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