In an attempt to probe the relationship between excitotoxicity and increases in intracellular calcium ([Ca2+]i), BAPTA-AM and its analogs were applied to cultured hippocampal neurons. Chelation of [Ca2+]i depressed and prolonged transient responses to glutamate and did not effect elevation of [Ca2+]i by prolonged exposure. This explains the inability of the chelators to prevent glutamate-induced toxicity.
Bibliographical noteFunding Information:
I would like to thank Dr. Steven M. Rothman for use of the photometer-based calcium ratioing system in his laboratory and Ms. Marta Fournier and Nancy Lancaster for preparation of the cultures. Support for these experiments was provided by NIH Grants NS19988 to S.M. Rothman and AG10034 to J.M.D.
- Calcium chelator
- Intracellular calcium