Effects of calcium chelators on intracellular calcium and excitotoxicity

Janet M. Dubinsky

doi:10.1016/0304-3940(93)90518-P

Effects of calcium chelators on intracellular calcium and excitotoxicity

Janet M. Dubinsky

Neuroscience

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

In an attempt to probe the relationship between excitotoxicity and increases in intracellular calcium ([Ca²⁺]_i), BAPTA-AM and its analogs were applied to cultured hippocampal neurons. Chelation of [Ca²⁺]_i depressed and prolonged transient responses to glutamate and did not effect elevation of [Ca²⁺]_i by prolonged exposure. This explains the inability of the chelators to prevent glutamate-induced toxicity.

Original language	English (US)
Pages (from-to)	129-132
Number of pages	4
Journal	Neuroscience Letters
Volume	150
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(93)90518-P
State	Published - Feb 19 1993

Bibliographical note

Funding Information:
I would like to thank Dr. Steven M. Rothman for use of the photometer-based calcium ratioing system in his laboratory and Ms. Marta Fournier and Nancy Lancaster for preparation of the cultures. Support for these experiments was provided by NIH Grants NS19988 to S.M. Rothman and AG10034 to J.M.D.

Keywords

Calcium chelator
Excitotoxicity
Fura-2
Glutamate
Intracellular calcium
Neurotoxicity

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N2 - In an attempt to probe the relationship between excitotoxicity and increases in intracellular calcium ([Ca2+]i), BAPTA-AM and its analogs were applied to cultured hippocampal neurons. Chelation of [Ca2+]i depressed and prolonged transient responses to glutamate and did not effect elevation of [Ca2+]i by prolonged exposure. This explains the inability of the chelators to prevent glutamate-induced toxicity.

AB - In an attempt to probe the relationship between excitotoxicity and increases in intracellular calcium ([Ca2+]i), BAPTA-AM and its analogs were applied to cultured hippocampal neurons. Chelation of [Ca2+]i depressed and prolonged transient responses to glutamate and did not effect elevation of [Ca2+]i by prolonged exposure. This explains the inability of the chelators to prevent glutamate-induced toxicity.

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KW - Glutamate

KW - Intracellular calcium

KW - Neurotoxicity

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Effects of calcium chelators on intracellular calcium and excitotoxicity

Abstract

Bibliographical note

Keywords

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