Effects of chronic ethanol consumption on male syrian hamster hepatic, microsomal mixed-function oxidases

Charlotte R. Elliott, Jagarlapudi S. Prasad, Alan D. Husby, Randy J. Ellingson, Jordan L. Holtzman, Daune L. Crankshaw

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Chronic alcohol consumption significantly increases the risk of drug interactions. We have described its effects on hamster microsomal monooxygenases. Male Syrian hamsters (85 g) were given 10% ethanol in water and food ad lib for up to 6 weeks. Microsomal electron transport components and metabolism of ethylmorphine, benzphetamine, aniline, and acetaminophen were measured. At 4 weeks, SDS-PAGE of ethanol microsomes showed an induced band with an Mr of 53,900 daltons and there was a 2-3 fold stimulation of aniline and acetaminophen metabolism. Cytochrome P-450 increase was not significant. For the six week period, Caloric intake (3 weeks, p<0.001), liquid consumption (3 weeks, p<0.05) and body weights (6 weeks, p<0.05) of ethanol animals were significantly greater than controls; kidney weights were significantly less (p<0.05). Ethanol consumption increased from 20% of the daily caloric intake (week 1) to 31% (week 6). Induction of specific substrate metabolism without apparent deleterious physiological changes establishes hamsters fed 10% ethanol in drinking water as a biochemical model for the study of chronic alcohol consumption and specific drug interactions.

Original languageEnglish (US)
Pages (from-to)17-22
Number of pages6
Issue number1
StatePublished - Jan 1 1985

Bibliographical note

Copyright 2014 Elsevier B.V., All rights reserved.


  • Alcohol consumption
  • Ethanol
  • Hamster
  • Microsomal oxidase


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