TY - JOUR
T1 - Effects of chronic ethanol drinking on the blood-brain barrier and ensuing neuronal toxicity in alcohol-preferring rats subjected to intraperitoneal LPS injection
AU - Singh, Ashok K
AU - Jiang, Yin
AU - Gupta, Shveta
AU - Benlhabib, Elhabib
PY - 2007/9
Y1 - 2007/9
N2 - Aims: Although alcohol drinking impairs the blood-brain barrier (BBB), the underlying mechanism is not fully understood. Thus, the effects of chronic ethanol drinking on the BBB were studied in vivo. Methods: Alcohol-preferring rats were given for 70 days free choice water and 15% ethanol. Then, they received LPS by i.p. injection. Efflux of [14C]sucrose or [14C]dextran was measured by their microinjection into the brain. Endothelial cells and neurons were isolated from the brain and analysed for mitogen-activated protein kinase (MAPK) and the tight-junction (TJ) protein phosphorylation, NFκB activation, mRNA levels of TJ proteins, inducible nitric oxide synthase, tumour necrosis factor α, interleukin-1 β (IL-1β), IL-10, CASPASE-8, and DNA damage. Results: LPS transiently increased [14C]sucrose efflux in water drinking, while it caused a lasting increase in [14C] sucrose and [14C]dextran efflux in ethanol-drinking rats. The time-course of changes in the TJ correlated with (i) an increase in extracellular signal-regulated kinase (ERK), p38mapk Jun-N-terminal Kinase (JNK), and TJ protein phosphorylation, (ii) RelA-p50 and p50-p50 activation, and (iii) a decrease in the TJ proteins' mRNA levels in endothelial cells and neurons. Apoptotic cells were detected in water drinking and LPS (WC-LPS) neurons at 24 h after LPS exposure. Neurons from Et-LPS rats did not exhibit apoptosis. Conclusions: LPS injection in WC-LPS rats transiently disrupted the BBB. Lack of JNK activation and CASPASE-8 may be responsible for lack of apoptosis in endothelial cells and vice versa in neurons. Chronic alcohol drinking in ethanol drinking and LPS (Et-LPS) rats augmented and dysregulated the LPS-induced BBB abnormalities but suppressed apoptosis in neurons.
AB - Aims: Although alcohol drinking impairs the blood-brain barrier (BBB), the underlying mechanism is not fully understood. Thus, the effects of chronic ethanol drinking on the BBB were studied in vivo. Methods: Alcohol-preferring rats were given for 70 days free choice water and 15% ethanol. Then, they received LPS by i.p. injection. Efflux of [14C]sucrose or [14C]dextran was measured by their microinjection into the brain. Endothelial cells and neurons were isolated from the brain and analysed for mitogen-activated protein kinase (MAPK) and the tight-junction (TJ) protein phosphorylation, NFκB activation, mRNA levels of TJ proteins, inducible nitric oxide synthase, tumour necrosis factor α, interleukin-1 β (IL-1β), IL-10, CASPASE-8, and DNA damage. Results: LPS transiently increased [14C]sucrose efflux in water drinking, while it caused a lasting increase in [14C] sucrose and [14C]dextran efflux in ethanol-drinking rats. The time-course of changes in the TJ correlated with (i) an increase in extracellular signal-regulated kinase (ERK), p38mapk Jun-N-terminal Kinase (JNK), and TJ protein phosphorylation, (ii) RelA-p50 and p50-p50 activation, and (iii) a decrease in the TJ proteins' mRNA levels in endothelial cells and neurons. Apoptotic cells were detected in water drinking and LPS (WC-LPS) neurons at 24 h after LPS exposure. Neurons from Et-LPS rats did not exhibit apoptosis. Conclusions: LPS injection in WC-LPS rats transiently disrupted the BBB. Lack of JNK activation and CASPASE-8 may be responsible for lack of apoptosis in endothelial cells and vice versa in neurons. Chronic alcohol drinking in ethanol drinking and LPS (Et-LPS) rats augmented and dysregulated the LPS-induced BBB abnormalities but suppressed apoptosis in neurons.
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U2 - 10.1093/alcalc/agl120
DO - 10.1093/alcalc/agl120
M3 - Article
C2 - 17341516
AN - SCOPUS:35349021105
SN - 0735-0414
VL - 42
SP - 385
EP - 399
JO - Alcohol and Alcoholism
JF - Alcohol and Alcoholism
IS - 5
ER -