Effects of galanin on smooth muscle and mucosa of porcine jejunum

David R. Brown; Keith R. Hildebrand; Ann M. Parsons; Giulio Soldani

doi:10.1016/0196-9781(90)90049-B

Effects of galanin on smooth muscle and mucosa of porcine jejunum

David R. Brown, Keith R. Hildebrand, Ann M. Parsons, Giulio Soldani

Chronic Disease Biology

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31 Scopus citations

Abstract

The enteric neuropeptide galanin (GAL) increased the amplitude of spontaneous contractions in longitudinally oriented muscle strips and inhibited short-circuit current (I_sc) elevations induced by transmural electrical stimulation (ES) of mucosal sheets from porcine jejunum in vitro. GAL-induced contractions (GAL EC₅₀ = 9 nmol/l) were maximally 25% of those elecited by 10 μmol/l carbamylcholine and remained unaffected by atropine, tetrodotoxin, or tachyphylaxis to substance P. The presynaptic Ca²⁺ channel blocker, omega-conotoxin (0.1 μmol/l), inhibited GAL-induced contractions by 66%. GAL attenuated mucosal I_sc elevations induced by ES with an IC₅₀ = 13 nmol/l and at 0.1 μmol/l produced rapi decreases in basal I_sc averaging 8±2 μA cm^-2 in 77% of tissues examined. The α-adrenoceptor blocker phentolamine or the opiate antagonist naloxone did not alter tissue I_sc responses to GAL. These results suggest that GAL modulates neuronal activity linked to secretomotor function in the porcine small intestine.

Original language	English (US)
Pages (from-to)	497-500
Number of pages	4
Journal	Peptides
Volume	11
Issue number	3
DOIs	https://doi.org/10.1016/0196-9781(90)90049-B
State	Published - 1990

Keywords

Contractility
Enteric nervous system
Galanin
Ion transport
Jejunum
Longitudinal muscle
Pig

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title = "Effects of galanin on smooth muscle and mucosa of porcine jejunum",

abstract = "The enteric neuropeptide galanin (GAL) increased the amplitude of spontaneous contractions in longitudinally oriented muscle strips and inhibited short-circuit current (Isc) elevations induced by transmural electrical stimulation (ES) of mucosal sheets from porcine jejunum in vitro. GAL-induced contractions (GAL EC50 = 9 nmol/l) were maximally 25% of those elecited by 10 μmol/l carbamylcholine and remained unaffected by atropine, tetrodotoxin, or tachyphylaxis to substance P. The presynaptic Ca2+ channel blocker, omega-conotoxin (0.1 μmol/l), inhibited GAL-induced contractions by 66%. GAL attenuated mucosal Isc elevations induced by ES with an IC50 = 13 nmol/l and at 0.1 μmol/l produced rapi decreases in basal Isc averaging 8±2 μA cm-2 in 77% of tissues examined. The α-adrenoceptor blocker phentolamine or the opiate antagonist naloxone did not alter tissue Isc responses to GAL. These results suggest that GAL modulates neuronal activity linked to secretomotor function in the porcine small intestine.",

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author = "Brown, {David R.} and Hildebrand, {Keith R.} and Parsons, {Ann M.} and Giulio Soldani",

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AU - Parsons, Ann M.

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AB - The enteric neuropeptide galanin (GAL) increased the amplitude of spontaneous contractions in longitudinally oriented muscle strips and inhibited short-circuit current (Isc) elevations induced by transmural electrical stimulation (ES) of mucosal sheets from porcine jejunum in vitro. GAL-induced contractions (GAL EC50 = 9 nmol/l) were maximally 25% of those elecited by 10 μmol/l carbamylcholine and remained unaffected by atropine, tetrodotoxin, or tachyphylaxis to substance P. The presynaptic Ca2+ channel blocker, omega-conotoxin (0.1 μmol/l), inhibited GAL-induced contractions by 66%. GAL attenuated mucosal Isc elevations induced by ES with an IC50 = 13 nmol/l and at 0.1 μmol/l produced rapi decreases in basal Isc averaging 8±2 μA cm-2 in 77% of tissues examined. The α-adrenoceptor blocker phentolamine or the opiate antagonist naloxone did not alter tissue Isc responses to GAL. These results suggest that GAL modulates neuronal activity linked to secretomotor function in the porcine small intestine.

KW - Contractility

KW - Enteric nervous system

KW - Galanin

KW - Ion transport

KW - Jejunum

KW - Longitudinal muscle

KW - Pig

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Effects of galanin on smooth muscle and mucosa of porcine jejunum

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