Abstract
The effects of nicotine and other cholinergic drugs on the initial incorporation of 32Pi, and [3H]glycerol into 1-phosphatidylinositol-4-phosphate(DPI)and 1-phosphatidylinsitol-3,4-bisphosphate (TPI) in the rat brain microsomal fraction were studied. Nicotine and eserine significantly decreased and mecamylamine increased [32P]- and [3H]TPI levels. Atropine had no effect on labeled TPI levels. Dose-effect studies for nicotine revealed that as little as 0.1 mg/kg of nicotine, i.p., significantly decreased the accumulation of labeled TPI. It was concluded that pharmacologically relevant and nonconvulsive doses of nicotine can markedly affect brain polyphosphoinositide synthesis and/or metabolism; the nature of the effects is consistent with theories suggesting that the conversion of TPI to DPI will release membrane lipid bound Ca2+ and, thus, increase membrane excitability.
Original language | English (US) |
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Pages (from-to) | 2519-2523 |
Number of pages | 5 |
Journal | Biochemical Pharmacology |
Volume | 27 |
Issue number | 21 |
DOIs | |
State | Published - 1978 |
Bibliographical note
Funding Information:Acknowledgements-Thea uthorsa cknowledgteh etech-nical assistancoef BarbaraH itzemanna nd thee ditorial and typing assistanceo f BarbaraH alperin and Kaye Welch. This study was supported in part by USPHS
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.