Abstract
OBJECTIVE To evaluate safety of stylet-in and stylet-out techniques for collection of CSF from the cisterna magna and to assess whether there were differences between techniques with regard to contamination of samples, sample quality, and efficiency of collection. ANIMALS 10 adult purpose-bred research Beagles. PROCEDURES A prospective crossover study was conducted. Preanesthetic physical and neurologic examinations and hematologic analyses were performed. Dogs were anesthetized, and collection of CSF samples from the cisterna magna by use of a stylet-in or stylet-out technique was performed. Two weeks later, samples were collected with the other sample collection technique. Samples of CSF were processed within 1 hour after collection. RESULTS Cellular debris was detected in higher numbers in stylet-in samples, although this did not affect sample quality. The stylet-out technique was performed more rapidly. No adverse effects were detected for either technique. CONCLUSIONS AND CLINICAL RELEVANCE Both techniques could be safely performed in healthy anesthetized dogs. The stylet-out technique was performed more rapidly and yielded a sample with less cellular debris. Both techniques can be used in clinical practice to yield CSF samples with good diagnostic quality.
Original language | English (US) |
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Pages (from-to) | 787-791 |
Number of pages | 5 |
Journal | American journal of veterinary research |
Volume | 80 |
Issue number | 8 |
DOIs | |
State | Published - Aug 2019 |
Bibliographical note
Funding Information:Supported by the Companion Animal Trust Fund, Atlantic Veterinary College, University of Prince Edward Island. The funding source did not have any involvement in the study design, data analysis and interpretation, or writing and publication of the manuscript. The authors thank Dr. Henrik Stryhn and Gabrielle Monteith for assistance with statistical analyses.
Funding Information:
Supported by the Companion Animal Trust Fund, Atlantic Veterinary College, University of Prince Edward Island. The funding source did not have any involvement in the study design, data analysis and interpretation, or writing and publication of the manuscript.
Publisher Copyright:
© 2019, American Veterinary Medical Association. All rights reserved.