Effects of temperature and stabilizer on the viability of a live attenuated avian metapneumovirus vaccine

M. A. Ramakrishnan; Binu T. Velayudhan; Senthilvelan Anantharaman; Sally L. Noll; David A. Halvorson; Kakambi V. Nagaraja; Sagar M. Goyal

doi:10.1637/7962-030107.1

Effects of temperature and stabilizer on the viability of a live attenuated avian metapneumovirus vaccine

M. A. Ramakrishnan, Binu T. Velayudhan, Senthilvelan Anantharaman, Sally L. Noll, David A. Halvorson, Kakambi V. Nagaraja, Sagar M. Goyal

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Abstract

A commercial live attenuated, freeze-dried avian metapneumovirus vaccine, Pneumomune^TM, was assessed for its viability at three different temperatures (5.6 C, 21 C, and 37 C). No significant reduction in virus titer was observed when the vaccine was stored at 5.6 C for a period of 24 hr. However, reductions in virus titer of 1 log10 and 2 log10 were observed after 24 hr at 21 C and 37 C, respectively. Batch-to-batch variation in virus reduction was also observed. The addition of a dye or a vaccine stabilizer to the vaccine preparation did not have any deleterious effect on the survival of vaccine virus.

Original language	English (US)
Pages (from-to)	979-981
Number of pages	3
Journal	Avian diseases
Volume	51
Issue number	4
DOIs	https://doi.org/10.1637/7962-030107.1
State	Published - Dec 2007

Keywords

Avian metapneumovirus
Dye
Stabilizer
Temperature
Vaccine
Viability

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title = "Effects of temperature and stabilizer on the viability of a live attenuated avian metapneumovirus vaccine",

abstract = "A commercial live attenuated, freeze-dried avian metapneumovirus vaccine, PneumomuneTM, was assessed for its viability at three different temperatures (5.6 C, 21 C, and 37 C). No significant reduction in virus titer was observed when the vaccine was stored at 5.6 C for a period of 24 hr. However, reductions in virus titer of 1 log10 and 2 log10 were observed after 24 hr at 21 C and 37 C, respectively. Batch-to-batch variation in virus reduction was also observed. The addition of a dye or a vaccine stabilizer to the vaccine preparation did not have any deleterious effect on the survival of vaccine virus.",

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author = "Ramakrishnan, {M. A.} and Velayudhan, {Binu T.} and Senthilvelan Anantharaman and Noll, {Sally L.} and Halvorson, {David A.} and Nagaraja, {Kakambi V.} and Goyal, {Sagar M.}",

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AU - Velayudhan, Binu T.

AU - Anantharaman, Senthilvelan

AU - Noll, Sally L.

AU - Halvorson, David A.

AU - Nagaraja, Kakambi V.

AU - Goyal, Sagar M.

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KW - Stabilizer

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Effects of temperature and stabilizer on the viability of a live attenuated avian metapneumovirus vaccine

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