Electron Microscopic Study of Corneal Epithelial-Endothelial Interactions in Organ Culture

Joseph W. Sassani, Thomas John, J. Douglas Cameron, Myron Yanoff, Ralph C. Eagle

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The interactions between rabbit corneal epithelium and endothelium were investigated in an organ culture model using scanning electron microscopy and correlative light microscopy. Following mechanical removal of corneal endothelium, corneal epithelium was observed to migrate across the cut surface of the corneal stroma and onto the denuded Descemet's membrane after 48 hours in organ culture. By 72 hours, a distinct line of contact between the migrated epithelium and endothelium was established. Following epithelialendothelial contact, no cellular migration occurred. Corneal endothelial inhibition of epithelial migration may be a major factor in the prevention of surface epithelial invasion and proliferation following surgery and trauma.

Original languageEnglish (US)
Pages (from-to)553-557
Number of pages5
JournalOphthalmology
Volume91
Issue number6
DOIs
StatePublished - 1984

Bibliographical note

Funding Information:
Supported in part by the Harry and Edith H. Hubschman Research Fund and Grant 5 T32 EY07041-03, National Eye Institute, National Institutes of Health.

Keywords

  • contact inhibition
  • corneal endothelium
  • corneal epithelium
  • organ culture
  • scanning electron microscopy

Fingerprint

Dive into the research topics of 'Electron Microscopic Study of Corneal Epithelial-Endothelial Interactions in Organ Culture'. Together they form a unique fingerprint.

Cite this