Electron Microscopic Study of Corneal Epithelial-Endothelial Interactions in Organ Culture

Joseph W. Sassani; Thomas John; J. Douglas Cameron; Myron Yanoff; Ralph C. Eagle

doi:10.1016/S0161-6420(84)34250-6

Electron Microscopic Study of Corneal Epithelial-Endothelial Interactions in Organ Culture

Joseph W. Sassani, Thomas John, J. Douglas Cameron, Myron Yanoff, Ralph C. Eagle

Research output: Contribution to journal › Article › peer-review

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Abstract

The interactions between rabbit corneal epithelium and endothelium were investigated in an organ culture model using scanning electron microscopy and correlative light microscopy. Following mechanical removal of corneal endothelium, corneal epithelium was observed to migrate across the cut surface of the corneal stroma and onto the denuded Descemet's membrane after 48 hours in organ culture. By 72 hours, a distinct line of contact between the migrated epithelium and endothelium was established. Following epithelialendothelial contact, no cellular migration occurred. Corneal endothelial inhibition of epithelial migration may be a major factor in the prevention of surface epithelial invasion and proliferation following surgery and trauma.

Original language	English (US)
Pages (from-to)	553-557
Number of pages	5
Journal	Ophthalmology
Volume	91
Issue number	6
DOIs	https://doi.org/10.1016/S0161-6420(84)34250-6
State	Published - 1984
Externally published	Yes

Bibliographical note

Funding Information:
Supported in part by the Harry and Edith H. Hubschman Research Fund and Grant 5 T32 EY07041-03, National Eye Institute, National Institutes of Health.

Keywords

contact inhibition
corneal endothelium
corneal epithelium
organ culture
scanning electron microscopy

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title = "Electron Microscopic Study of Corneal Epithelial-Endothelial Interactions in Organ Culture",

abstract = "The interactions between rabbit corneal epithelium and endothelium were investigated in an organ culture model using scanning electron microscopy and correlative light microscopy. Following mechanical removal of corneal endothelium, corneal epithelium was observed to migrate across the cut surface of the corneal stroma and onto the denuded Descemet's membrane after 48 hours in organ culture. By 72 hours, a distinct line of contact between the migrated epithelium and endothelium was established. Following epithelialendothelial contact, no cellular migration occurred. Corneal endothelial inhibition of epithelial migration may be a major factor in the prevention of surface epithelial invasion and proliferation following surgery and trauma.",

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author = "Sassani, {Joseph W.} and Thomas John and Cameron, {J. Douglas} and Myron Yanoff and Eagle, {Ralph C.}",

note = "Funding Information: Supported in part by the Harry and Edith H. Hubschman Research Fund and Grant 5 T32 EY07041-03, National Eye Institute, National Institutes of Health.",

year = "1984",

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language = "English (US)",

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AU - Sassani, Joseph W.

AU - John, Thomas

AU - Cameron, J. Douglas

AU - Yanoff, Myron

AU - Eagle, Ralph C.

N1 - Funding Information: Supported in part by the Harry and Edith H. Hubschman Research Fund and Grant 5 T32 EY07041-03, National Eye Institute, National Institutes of Health.

PY - 1984

Y1 - 1984

N2 - The interactions between rabbit corneal epithelium and endothelium were investigated in an organ culture model using scanning electron microscopy and correlative light microscopy. Following mechanical removal of corneal endothelium, corneal epithelium was observed to migrate across the cut surface of the corneal stroma and onto the denuded Descemet's membrane after 48 hours in organ culture. By 72 hours, a distinct line of contact between the migrated epithelium and endothelium was established. Following epithelialendothelial contact, no cellular migration occurred. Corneal endothelial inhibition of epithelial migration may be a major factor in the prevention of surface epithelial invasion and proliferation following surgery and trauma.

AB - The interactions between rabbit corneal epithelium and endothelium were investigated in an organ culture model using scanning electron microscopy and correlative light microscopy. Following mechanical removal of corneal endothelium, corneal epithelium was observed to migrate across the cut surface of the corneal stroma and onto the denuded Descemet's membrane after 48 hours in organ culture. By 72 hours, a distinct line of contact between the migrated epithelium and endothelium was established. Following epithelialendothelial contact, no cellular migration occurred. Corneal endothelial inhibition of epithelial migration may be a major factor in the prevention of surface epithelial invasion and proliferation following surgery and trauma.

KW - contact inhibition

KW - corneal endothelium

KW - corneal epithelium

KW - organ culture

KW - scanning electron microscopy

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Electron Microscopic Study of Corneal Epithelial-Endothelial Interactions in Organ Culture

Abstract

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Keywords

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