Electrophoretic separation of proteins via complexation with a polyelectrolyte

E. M. Baskin, B. I. Shklovskii, G. V. Zilberstein

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4 Scopus citations

Abstract

We suggest to augment standard isoelectric focusing for separation of proteins in a gradient of pH by a similar focusing in the presence of a strongly charged polyelectrolyte (PE). Proteins which have the same isoelectric point but different "hidden" charges of both signs in this point make complexes with PE, which focus in different pH. This is a result of charge inversion of such proteins by adsorbed PE molecules, which is sensitive to the hidden charge. Hence, the hidden charge is a new separation parameter.

Original languageEnglish (US)
Pages (from-to)313-320
Number of pages8
JournalPhysica A: Statistical Mechanics and its Applications
Volume317
Issue number3-4
DOIs
StatePublished - Jan 15 2003

Bibliographical note

Funding Information:
We are grateful to A.Yu. Grosberg and T.T. Nguyen, for useful discussions. B.I. Shklovskii was supported by NSF Grant DMR-9985785. E.M. Baskin thanks the Center for Absorption in Science, Israel Ministry of Absorption for financial support.

Keywords

  • Charge inversion
  • Isoelectric focusing
  • Proteomics

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