Electrophoretic separation of proteins via complexation with a polyelectrolyte

E. M. Baskin; B. I. Shklovskii; G. V. Zilberstein

doi:10.1016/S0378-4371(02)01334-1

Electrophoretic separation of proteins via complexation with a polyelectrolyte

E. M. Baskin, B. I. Shklovskii, G. V. Zilberstein

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Abstract

We suggest to augment standard isoelectric focusing for separation of proteins in a gradient of pH by a similar focusing in the presence of a strongly charged polyelectrolyte (PE). Proteins which have the same isoelectric point but different "hidden" charges of both signs in this point make complexes with PE, which focus in different pH. This is a result of charge inversion of such proteins by adsorbed PE molecules, which is sensitive to the hidden charge. Hence, the hidden charge is a new separation parameter.

Original language	English (US)
Pages (from-to)	313-320
Number of pages	8
Journal	Physica A: Statistical Mechanics and its Applications
Volume	317
Issue number	3-4
DOIs	https://doi.org/10.1016/S0378-4371(02)01334-1
State	Published - Jan 15 2003

Bibliographical note

Funding Information:
We are grateful to A.Yu. Grosberg and T.T. Nguyen, for useful discussions. B.I. Shklovskii was supported by NSF Grant DMR-9985785. E.M. Baskin thanks the Center for Absorption in Science, Israel Ministry of Absorption for financial support.

Keywords

Charge inversion
Isoelectric focusing
Proteomics

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N2 - We suggest to augment standard isoelectric focusing for separation of proteins in a gradient of pH by a similar focusing in the presence of a strongly charged polyelectrolyte (PE). Proteins which have the same isoelectric point but different "hidden" charges of both signs in this point make complexes with PE, which focus in different pH. This is a result of charge inversion of such proteins by adsorbed PE molecules, which is sensitive to the hidden charge. Hence, the hidden charge is a new separation parameter.

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KW - Isoelectric focusing

KW - Proteomics

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Electrophoretic separation of proteins via complexation with a polyelectrolyte

Abstract

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Keywords

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