TY - JOUR
T1 - Engineering a Native Inducible Expression System in Shewanella oneidensis to Control Extracellular Electron Transfer
AU - West, Elizabeth A.
AU - Jain, Abhiney
AU - Gralnick, Jeffrey A.
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/9/15
Y1 - 2017/9/15
N2 - Shewanella oneidensis MR-1 is a model organism for understanding extracellular electron transport, in which cells transfer intracellular electrons to an extracellular terminal electron acceptor such as insoluble minerals or poised electrodes. Biotechnological applications exploiting the respiratory capabilities of Shewanella species have led to their proposed use in wastewater treatment, bioremediation, and remote sensors. Transcriptional regulation tools can be used to rationally engineer S. oneidensis, optimizing performance in biotechnological applications, introducing new capabilities, or investigating physiology. Engineered gene expression in S. oneidensis has primarily involved the use of foreign regulatory systems from Escherichia coli. Here we characterize a native S. oneidensis pathway that can be used to induce gene expression with trimethylamine N-oxide, then engineer strains in which extracellular electron transfer is controlled by this compound. The ability to induce this pathway was assessed by measuring iron reduction over time and by analyzing anodic current produced by cells grown in bioreactors.
AB - Shewanella oneidensis MR-1 is a model organism for understanding extracellular electron transport, in which cells transfer intracellular electrons to an extracellular terminal electron acceptor such as insoluble minerals or poised electrodes. Biotechnological applications exploiting the respiratory capabilities of Shewanella species have led to their proposed use in wastewater treatment, bioremediation, and remote sensors. Transcriptional regulation tools can be used to rationally engineer S. oneidensis, optimizing performance in biotechnological applications, introducing new capabilities, or investigating physiology. Engineered gene expression in S. oneidensis has primarily involved the use of foreign regulatory systems from Escherichia coli. Here we characterize a native S. oneidensis pathway that can be used to induce gene expression with trimethylamine N-oxide, then engineer strains in which extracellular electron transfer is controlled by this compound. The ability to induce this pathway was assessed by measuring iron reduction over time and by analyzing anodic current produced by cells grown in bioreactors.
KW - extracellular electron transport
KW - regulation
KW - trimethylamine N-oxide (TMAO)
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U2 - 10.1021/acssynbio.6b00349
DO - 10.1021/acssynbio.6b00349
M3 - Article
C2 - 28562022
AN - SCOPUS:85029467652
SN - 2161-5063
VL - 6
SP - 1627
EP - 1634
JO - ACS Synthetic Biology
JF - ACS Synthetic Biology
IS - 9
ER -