TY - JOUR
T1 - Evaluation of bacteriologic culture of pooled fecal samples for detection of Mycobacterium paratuberculosis
AU - Wells, Scott J.
AU - Whitlock, Robert H.
AU - Lindeman, Cynthia J.
AU - Fyock, Terry
PY - 2002/8/1
Y1 - 2002/8/1
N2 - Objectives - To compare sensitivity of several methods of bacteriologic culture of pooled bovine fecal samples for detection of Mycobacterium paratuberculosis and evaluate homogeneity in number of M paratuberculosis in pooled fecal samples. Sample Population - Feces from 10 dairy cows that shed M paratuberculosis at various concentrations and 1 dairy cow known to be free of infection with M paratuberculosis. Procedure - 5 fecal pooling methods, 2 culture methods, and 2 pool sizes were evaluated. Each pooled sample contained 1 infected sample and 4 or 9 uninfected samples. Results - Sensitivity of detection of M paratuberculosis was greater with smaller pool size (5 vs 10 samples/pool). Detection sensitivity was also associated with concentration of bacteria in the infected sample. Results indicated that, compared with concurrent bacterial culture of individual infected samples, 37 to 44% of pooled samples with low bacterial concentrations yielded positive culture results and 94% of pooled samples with high bacterial concentrations yielded positive results. Conclusions and Clinical Relevance - Bacteriologic culture of pooled fecal samples may provide a valid and cost-effective method of detecting M paratuberculosis infection in cattle herds.
AB - Objectives - To compare sensitivity of several methods of bacteriologic culture of pooled bovine fecal samples for detection of Mycobacterium paratuberculosis and evaluate homogeneity in number of M paratuberculosis in pooled fecal samples. Sample Population - Feces from 10 dairy cows that shed M paratuberculosis at various concentrations and 1 dairy cow known to be free of infection with M paratuberculosis. Procedure - 5 fecal pooling methods, 2 culture methods, and 2 pool sizes were evaluated. Each pooled sample contained 1 infected sample and 4 or 9 uninfected samples. Results - Sensitivity of detection of M paratuberculosis was greater with smaller pool size (5 vs 10 samples/pool). Detection sensitivity was also associated with concentration of bacteria in the infected sample. Results indicated that, compared with concurrent bacterial culture of individual infected samples, 37 to 44% of pooled samples with low bacterial concentrations yielded positive culture results and 94% of pooled samples with high bacterial concentrations yielded positive results. Conclusions and Clinical Relevance - Bacteriologic culture of pooled fecal samples may provide a valid and cost-effective method of detecting M paratuberculosis infection in cattle herds.
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U2 - 10.2460/ajvr.2002.63.1207
DO - 10.2460/ajvr.2002.63.1207
M3 - Article
C2 - 12171178
AN - SCOPUS:0036701559
SN - 0002-9645
VL - 63
SP - 1207
EP - 1211
JO - American journal of veterinary research
JF - American journal of veterinary research
IS - 8
ER -