Evidence of cytocidal effects of Pasteurella haemolytica on bovine peripheral blood mononuclear leukocytes.

K. L. Kaehler, R. J. Markam, C. C. Muscoplat, D. W. Johnson

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Pasteurella haemolytica had a pronounced cytocidal effect on bovine mononuclear leukocytes. Mononuclear leukocytes separated from venous blood on Ficoll-diatrizoate and incubated with either P haemolytica or Escherichia coli showed a significantly greater percentage cell death than did cells incubated with Staphylococcus epidermidis or controls. Neither heat-killed nor x-irradiated P haemolytica caused a significant percentage cell death. The cytotoxic potential of P haemolytica was dose related in that cell death among mononuclear leukocytes increased as the ratio of bacteria-to-cells increased. Enriched monocyte cultures showed a higher and less variable rate of cell death than either enriched lymphocyte or mixed leukocyte cultures.

Original languageEnglish (US)
Pages (from-to)1690-1693
Number of pages4
JournalAmerican journal of veterinary research
Volume41
Issue number10
StatePublished - Oct 1 1980

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