Evidence that the yeast spindle assembly checkpoint has a target other than the anaphase promoting complex.

Catherine A. Andrews, Laura A. Díaz-Martínez, Juan F. Giménez-Abián, Vincent Guacci, Amit C. Vas, Duncan J. Clarke

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The spindle assembly checkpoint monitors biorientation of chromosomes on the metaphase spindle and inhibits the Anaphase Promoting Complex (APC) specificity factor Cdc20. If APC-Cdc20 is the sole target of the spindle checkpoint, then cells lacking APC and its targets, B-type cyclin and securin, would lack spindle checkpoint function. We tested this hypothesis in yeast cells that are APC-null. Surprisingly, we find that such yeast cells are able to activate the spindle assembly checkpoint, delaying cell cycle progression in G2/M phase. These data suggest that the spindle checkpoint has a non-APC target that can restrain anaphase onset.

Original languageEnglish (US)
Pages (from-to)1555-1557
Number of pages3
JournalCell Cycle
Volume4
Issue number11
DOIs
StatePublished - Nov 2005

Keywords

  • Anaphase
  • Anaphase promoting complex
  • APC
  • Bub1
  • Cdc20
  • Cdh1
  • Clb5
  • Cyclosome
  • Mad2
  • Nocodazole
  • Pds1
  • Securin
  • Sic1
  • Spindle checkpoint

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