Evidence that the yeast spindle assembly checkpoint has a target other than the anaphase promoting complex.

Catherine A. Andrews; Laura A. Díaz-Martínez; Juan F. Giménez-Abián; Vincent Guacci; Amit C. Vas; Duncan J. Clarke

doi:10.4161/cc.4.11.2144

Evidence that the yeast spindle assembly checkpoint has a target other than the anaphase promoting complex.

Catherine A. Andrews, Laura A. Díaz-Martínez, Juan F. Giménez-Abián, Vincent Guacci, Amit C. Vas, Duncan J. Clarke

Genetics, Cell Biology and Development (TMED)

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The spindle assembly checkpoint monitors biorientation of chromosomes on the metaphase spindle and inhibits the Anaphase Promoting Complex (APC) specificity factor Cdc20. If APC-Cdc20 is the sole target of the spindle checkpoint, then cells lacking APC and its targets, B-type cyclin and securin, would lack spindle checkpoint function. We tested this hypothesis in yeast cells that are APC-null. Surprisingly, we find that such yeast cells are able to activate the spindle assembly checkpoint, delaying cell cycle progression in G₂/M phase. These data suggest that the spindle checkpoint has a non-APC target that can restrain anaphase onset.

Original language	English (US)
Pages (from-to)	1555-1557
Number of pages	3
Journal	Cell Cycle
Volume	4
Issue number	11
DOIs	https://doi.org/10.4161/cc.4.11.2144
State	Published - Nov 2005

Keywords

Anaphase
Anaphase promoting complex
APC
Bub1
Cdc20
Cdh1
Clb5
Cyclosome
Mad2
Nocodazole
Pds1
Securin
Sic1
Spindle checkpoint

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title = "Evidence that the yeast spindle assembly checkpoint has a target other than the anaphase promoting complex.",

abstract = "The spindle assembly checkpoint monitors biorientation of chromosomes on the metaphase spindle and inhibits the Anaphase Promoting Complex (APC) specificity factor Cdc20. If APC-Cdc20 is the sole target of the spindle checkpoint, then cells lacking APC and its targets, B-type cyclin and securin, would lack spindle checkpoint function. We tested this hypothesis in yeast cells that are APC-null. Surprisingly, we find that such yeast cells are able to activate the spindle assembly checkpoint, delaying cell cycle progression in G2/M phase. These data suggest that the spindle checkpoint has a non-APC target that can restrain anaphase onset.",

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AU - Andrews, Catherine A.

AU - Díaz-Martínez, Laura A.

AU - Giménez-Abián, Juan F.

AU - Guacci, Vincent

AU - Vas, Amit C.

AU - Clarke, Duncan J.

PY - 2005/11

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AB - The spindle assembly checkpoint monitors biorientation of chromosomes on the metaphase spindle and inhibits the Anaphase Promoting Complex (APC) specificity factor Cdc20. If APC-Cdc20 is the sole target of the spindle checkpoint, then cells lacking APC and its targets, B-type cyclin and securin, would lack spindle checkpoint function. We tested this hypothesis in yeast cells that are APC-null. Surprisingly, we find that such yeast cells are able to activate the spindle assembly checkpoint, delaying cell cycle progression in G2/M phase. These data suggest that the spindle checkpoint has a non-APC target that can restrain anaphase onset.

KW - Anaphase

KW - Anaphase promoting complex

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KW - Cdc20

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KW - Cyclosome

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KW - Nocodazole

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KW - Securin

KW - Sic1

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ER -

Evidence that the yeast spindle assembly checkpoint has a target other than the anaphase promoting complex.

Abstract

Keywords

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