Excess neutrophil infiltration during cytomegalovirus brain infection of interleukin-10-deficient mice

Manohar B. Mutnal; Maxim C Cheeran; Shuxian Hu; Morgan R. Little; James R Lokensgard

doi:10.1016/j.jneuroim.2010.06.020

Excess neutrophil infiltration during cytomegalovirus brain infection of interleukin-10-deficient mice

Manohar B. Mutnal, Maxim C Cheeran, Shuxian Hu, Morgan R. Little, James R Lokensgard

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Abstract

Wild-type mice control murine cytomegalovirus (MCMV) brain infection, but identical infection is lethal to animals deficient in interleukin (IL)-10. Here, we report that MCMV-infected IL-10 knockout (KO) mice displayed a marked increase in neutrophil infiltration into the infected, IL-10-deficient brain when compared to wild-type animals. Enhanced microglial cell activation, determined by MHC class II up-regulation, overexpression of CXCL2, and elevated P-selectin mRNA levels were observed. In vivo blocking of CXCL2 attenuated neutrophil infiltration and significantly improved the outcome of infection. Collectively, these data indicate that the absence of IL-10 results in pathologic neutrophil infiltration into MCMV-infected brains.

Original language	English (US)
Pages (from-to)	101-110
Number of pages	10
Journal	Journal of Neuroimmunology
Volume	227
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2010.06.020
State	Published - Oct 2010

Bibliographical note

Funding Information:
This project was supported by Award Number R01 NS-038836 from the National Institute of Neurological Disorders and Stroke .

Keywords

CXCL2
IL-10
Microglia
Neutrophil

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title = "Excess neutrophil infiltration during cytomegalovirus brain infection of interleukin-10-deficient mice",

abstract = "Wild-type mice control murine cytomegalovirus (MCMV) brain infection, but identical infection is lethal to animals deficient in interleukin (IL)-10. Here, we report that MCMV-infected IL-10 knockout (KO) mice displayed a marked increase in neutrophil infiltration into the infected, IL-10-deficient brain when compared to wild-type animals. Enhanced microglial cell activation, determined by MHC class II up-regulation, overexpression of CXCL2, and elevated P-selectin mRNA levels were observed. In vivo blocking of CXCL2 attenuated neutrophil infiltration and significantly improved the outcome of infection. Collectively, these data indicate that the absence of IL-10 results in pathologic neutrophil infiltration into MCMV-infected brains.",

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note = "Funding Information: This project was supported by Award Number R01 NS-038836 from the National Institute of Neurological Disorders and Stroke .",

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AU - Hu, Shuxian

AU - Little, Morgan R.

AU - Lokensgard, James R

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AB - Wild-type mice control murine cytomegalovirus (MCMV) brain infection, but identical infection is lethal to animals deficient in interleukin (IL)-10. Here, we report that MCMV-infected IL-10 knockout (KO) mice displayed a marked increase in neutrophil infiltration into the infected, IL-10-deficient brain when compared to wild-type animals. Enhanced microglial cell activation, determined by MHC class II up-regulation, overexpression of CXCL2, and elevated P-selectin mRNA levels were observed. In vivo blocking of CXCL2 attenuated neutrophil infiltration and significantly improved the outcome of infection. Collectively, these data indicate that the absence of IL-10 results in pathologic neutrophil infiltration into MCMV-infected brains.

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Excess neutrophil infiltration during cytomegalovirus brain infection of interleukin-10-deficient mice

Abstract

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Keywords

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