Expression of an insecticidal fern protein in cotton protects against whitefly

Anoop Kumar Shukla; Santosh Kumar Upadhyay; Manisha Mishra; Sharad Saurabh; Rahul Singh; Harpal Singh; Nidhi Thakur; Preeti Rai; Paras Pandey; Aradhana L. Hans; Subhi Srivastava; Vikram Rajapure; Sunil Kumar Yadav; Mithlesh Kumar Singh; Jitendra Kumar; K. Chandrashekar; Praveen C. Verma; Ajit Pratap Singh; K. N. Nair; Smrati Bhadauria; Muhammad Wahajuddin; Sarika Singh; Sharad Sharma; Omkar; Ram Sanmukh Upadhyay; Shirish A. Ranade; Rakesh Tuli; Pradhyumna Kumar Singh

doi:10.1038/nbt.3665

Expression of an insecticidal fern protein in cotton protects against whitefly

Anoop Kumar Shukla, Santosh Kumar Upadhyay, Manisha Mishra, Sharad Saurabh, Rahul Singh, Harpal Singh, Nidhi Thakur, Preeti Rai, Paras Pandey, Aradhana L. Hans, Subhi Srivastava, Vikram Rajapure, Sunil Kumar Yadav, Mithlesh Kumar Singh, Jitendra Kumar, K. Chandrashekar, Praveen C. Verma, Ajit Pratap Singh, K. N. Nair, Smrati BhadauriaMuhammad Wahajuddin, Sarika Singh, Sharad Sharma, Omkar, Ram Sanmukh Upadhyay, Shirish A. Ranade, Rakesh Tuli, Pradhyumna Kumar Singh

Research output: Contribution to journal › Article › peer-review

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Abstract

Whitefly (Bemisia tabaci) damages field crops by sucking sap and transmitting viral diseases. None of the insecticidal proteins used in genetically modified (GM) crop plants to date are effective against whitefly. We report the identification of a protein (Tma12) from an edible fern, Tectaria macrodonta (Fee) C. Chr., that is insecticidal to whitefly (median lethal concentration = 1.49 μg/ml in in vitro feeding assays) and interferes with its life cycle at sublethal doses. Transgenic cotton lines that express Tma12 at ∼0.01% of total soluble leaf protein were resistant to whitefly infestation in contained field trials, with no detectable yield penalty. The transgenic cotton lines were also protected from whitefly-borne cotton leaf curl viral disease. Rats fed Tma12 showed no detectable histological or biochemical changes, and this, together with the predicted absence of allergenic domains in Tma12, indicates that Tma12 might be well suited for deployment in GM crops to control whitefly and the viruses it carries.

Original language	English (US)
Pages (from-to)	1046-1051
Number of pages	6
Journal	Nature biotechnology
Volume	34
Issue number	10
DOIs	https://doi.org/10.1038/nbt.3665
State	Published - Oct 1 2016
Externally published	Yes

Bibliographical note

Funding Information:
A.K.S., S.K.U., R.S., M.M., S. Saurabh, H.S., N.T., P.P., A.L.H. and V.R. are supported by CSIR. P.R. and S.K.Y. are supported by the Indian Council of Medical Research. S. Srivastava is supported by the University Grant Commission. R.T. is supported by a Department of Science and Technology (DST) JC Bose National Fellowship.

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Shukla, A. K., Upadhyay, S. K., Mishra, M., Saurabh, S., Singh, R., Singh, H., Thakur, N., Rai, P., Pandey, P., Hans, A. L., Srivastava, S., Rajapure, V., Yadav, S. K., Singh, M. K., Kumar, J., Chandrashekar, K., Verma, P. C., Singh, A. P., Nair, K. N., ... Singh, P. K. (2016). Expression of an insecticidal fern protein in cotton protects against whitefly. Nature biotechnology, 34(10), 1046-1051. https://doi.org/10.1038/nbt.3665

Shukla, AK, Upadhyay, SK, Mishra, M, Saurabh, S, Singh, R, Singh, H, Thakur, N, Rai, P, Pandey, P, Hans, AL, Srivastava, S, Rajapure, V, Yadav, SK, Singh, MK, Kumar, J, Chandrashekar, K, Verma, PC, Singh, AP, Nair, KN, Bhadauria, S, Wahajuddin, M, Singh, S, Sharma, S, Omkar, Upadhyay, RS, Ranade, SA, Tuli, R & Singh, PK 2016, 'Expression of an insecticidal fern protein in cotton protects against whitefly', Nature biotechnology, vol. 34, no. 10, pp. 1046-1051. https://doi.org/10.1038/nbt.3665

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abstract = "Whitefly (Bemisia tabaci) damages field crops by sucking sap and transmitting viral diseases. None of the insecticidal proteins used in genetically modified (GM) crop plants to date are effective against whitefly. We report the identification of a protein (Tma12) from an edible fern, Tectaria macrodonta (Fee) C. Chr., that is insecticidal to whitefly (median lethal concentration = 1.49 μg/ml in in vitro feeding assays) and interferes with its life cycle at sublethal doses. Transgenic cotton lines that express Tma12 at ∼0.01% of total soluble leaf protein were resistant to whitefly infestation in contained field trials, with no detectable yield penalty. The transgenic cotton lines were also protected from whitefly-borne cotton leaf curl viral disease. Rats fed Tma12 showed no detectable histological or biochemical changes, and this, together with the predicted absence of allergenic domains in Tma12, indicates that Tma12 might be well suited for deployment in GM crops to control whitefly and the viruses it carries.",

author = "Shukla, {Anoop Kumar} and Upadhyay, {Santosh Kumar} and Manisha Mishra and Sharad Saurabh and Rahul Singh and Harpal Singh and Nidhi Thakur and Preeti Rai and Paras Pandey and Hans, {Aradhana L.} and Subhi Srivastava and Vikram Rajapure and Yadav, {Sunil Kumar} and Singh, {Mithlesh Kumar} and Jitendra Kumar and K. Chandrashekar and Verma, {Praveen C.} and Singh, {Ajit Pratap} and Nair, {K. N.} and Smrati Bhadauria and Muhammad Wahajuddin and Sarika Singh and Sharad Sharma and Omkar and Upadhyay, {Ram Sanmukh} and Ranade, {Shirish A.} and Rakesh Tuli and Singh, {Pradhyumna Kumar}",

note = "Funding Information: A.K.S., S.K.U., R.S., M.M., S. Saurabh, H.S., N.T., P.P., A.L.H. and V.R. are supported by CSIR. P.R. and S.K.Y. are supported by the Indian Council of Medical Research. S. Srivastava is supported by the University Grant Commission. R.T. is supported by a Department of Science and Technology (DST) JC Bose National Fellowship.",

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AU - Shukla, Anoop Kumar

AU - Upadhyay, Santosh Kumar

AU - Mishra, Manisha

AU - Saurabh, Sharad

AU - Singh, Rahul

AU - Singh, Harpal

AU - Thakur, Nidhi

AU - Rai, Preeti

AU - Pandey, Paras

AU - Hans, Aradhana L.

AU - Srivastava, Subhi

AU - Rajapure, Vikram

AU - Yadav, Sunil Kumar

AU - Singh, Mithlesh Kumar

AU - Kumar, Jitendra

AU - Chandrashekar, K.

AU - Verma, Praveen C.

AU - Singh, Ajit Pratap

AU - Nair, K. N.

AU - Bhadauria, Smrati

AU - Wahajuddin, Muhammad

AU - Singh, Sarika

AU - Sharma, Sharad

AU - Omkar,

AU - Upadhyay, Ram Sanmukh

AU - Ranade, Shirish A.

AU - Tuli, Rakesh

AU - Singh, Pradhyumna Kumar

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PY - 2016/10/1

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N2 - Whitefly (Bemisia tabaci) damages field crops by sucking sap and transmitting viral diseases. None of the insecticidal proteins used in genetically modified (GM) crop plants to date are effective against whitefly. We report the identification of a protein (Tma12) from an edible fern, Tectaria macrodonta (Fee) C. Chr., that is insecticidal to whitefly (median lethal concentration = 1.49 μg/ml in in vitro feeding assays) and interferes with its life cycle at sublethal doses. Transgenic cotton lines that express Tma12 at ∼0.01% of total soluble leaf protein were resistant to whitefly infestation in contained field trials, with no detectable yield penalty. The transgenic cotton lines were also protected from whitefly-borne cotton leaf curl viral disease. Rats fed Tma12 showed no detectable histological or biochemical changes, and this, together with the predicted absence of allergenic domains in Tma12, indicates that Tma12 might be well suited for deployment in GM crops to control whitefly and the viruses it carries.

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Expression of an insecticidal fern protein in cotton protects against whitefly

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