Expression of fluorescent cyclotides using protein trans-splicing for easy monitoring of cyclotide-protein interactions

Krishnappa Jagadish, Radhika Borra, Vanessa Lacey, Subhabrata Majumder, Alexander Shekhtman, Lei Wang, Julio A. Camarero

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Going in circles: Expression of cyclotides containing non-natural amino acids (see scheme; gray sphere) inside live bacterial cells was accomplished using a highly efficient split intein in combination with nonsense codon suppressor tRNA technology. Cyclotides containing p-azidophenylalanine can be expressed in live bacterial cells and easily labeled using copper-free click chemistry to monitor the cyclotide-protein interactions.

Original languageEnglish (US)
Pages (from-to)3126-3131
Number of pages6
JournalAngewandte Chemie - International Edition
Volume52
Issue number11
DOIs
StatePublished - Mar 11 2013

Keywords

  • circular proteins
  • click chemistry
  • cyclotides
  • non-natural amino acids
  • protein engineering

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