Expression of fluorescent cyclotides using protein trans-splicing for easy monitoring of cyclotide-protein interactions

Krishnappa Jagadish; Radhika Borra; Vanessa Lacey; Subhabrata Majumder; Alexander Shekhtman; Lei Wang; Julio A. Camarero

doi:10.1002/anie.201209219

Expression of fluorescent cyclotides using protein trans-splicing for easy monitoring of cyclotide-protein interactions

Krishnappa Jagadish, Radhika Borra, Vanessa Lacey, Subhabrata Majumder, Alexander Shekhtman, Lei Wang, Julio A. Camarero

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Going in circles: Expression of cyclotides containing non-natural amino acids (see scheme; gray sphere) inside live bacterial cells was accomplished using a highly efficient split intein in combination with nonsense codon suppressor tRNA technology. Cyclotides containing p-azidophenylalanine can be expressed in live bacterial cells and easily labeled using copper-free click chemistry to monitor the cyclotide-protein interactions.

Original language	English (US)
Pages (from-to)	3126-3131
Number of pages	6
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	11
DOIs	https://doi.org/10.1002/anie.201209219
State	Published - Mar 11 2013
Externally published	Yes

Keywords

circular proteins
click chemistry
cyclotides
non-natural amino acids
protein engineering

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abstract = "Going in circles: Expression of cyclotides containing non-natural amino acids (see scheme; gray sphere) inside live bacterial cells was accomplished using a highly efficient split intein in combination with nonsense codon suppressor tRNA technology. Cyclotides containing p-azidophenylalanine can be expressed in live bacterial cells and easily labeled using copper-free click chemistry to monitor the cyclotide-protein interactions.",

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AU - Jagadish, Krishnappa

AU - Borra, Radhika

AU - Lacey, Vanessa

AU - Majumder, Subhabrata

AU - Shekhtman, Alexander

AU - Wang, Lei

AU - Camarero, Julio A.

PY - 2013/3/11

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AB - Going in circles: Expression of cyclotides containing non-natural amino acids (see scheme; gray sphere) inside live bacterial cells was accomplished using a highly efficient split intein in combination with nonsense codon suppressor tRNA technology. Cyclotides containing p-azidophenylalanine can be expressed in live bacterial cells and easily labeled using copper-free click chemistry to monitor the cyclotide-protein interactions.

KW - circular proteins

KW - click chemistry

KW - cyclotides

KW - non-natural amino acids

KW - protein engineering

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Expression of fluorescent cyclotides using protein trans-splicing for easy monitoring of cyclotide-protein interactions

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