Extracellular Amino Acid Concentrations in the Dorsal Spinal Cord of Freely Moving Rats Following Veratridine and Nociceptive Stimulation

Stephen R. Skilling, David H. Smullin, Al J Beitz, Alice A Larson

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Abstract

In vivo microdialysis was used to sample extracellular concentrations of amino acids in the dorsal lumbar spinal cord of freely moving rats. Changes in the extracellular concentrations of amino acids were measured in response to infusion of veratridine (180 μM), a sodium channel activator, as well as during acute noxious stimulation by an injection of 5% formalin into the metatarsal region of the hindleg. Veratridine produced a tetrodotoxin (TTX)‐sensitive increase in the extracellular concentration of Glu. Concentrations of Asp, taurine, Ala, Asn, and Gly were not significantly elevated following veratridine stimulation. In‐tradermal injection of formalin produced a TTX‐sensitive increase in Asp concentration and a non‐TTX‐sensitive increase in Glu concentration. These data support the hypothesis that Glu and Asp are dorsal horn neurotransmitters involved in nociception.

Original languageEnglish (US)
Pages (from-to)127-132
Number of pages6
JournalJournal of Neurochemistry
Volume51
Issue number1
DOIs
StatePublished - Jul 1988

Keywords

  • Amino acids
  • Aspartate
  • Glu‐tamate
  • Microdialysis
  • Nociception
  • Spinal cord

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