TY - JOUR
T1 - Force spectroscopy of single DNA and RNA molecules
AU - Williams, Mark C.
AU - Rouzina, Ioulia
PY - 2002/6/1
Y1 - 2002/6/1
N2 - Experiments in which single molecules of RNA and DNA are stretched, and the resulting force as a function of extension is measured have yielded new information about the physical, chemical and biological properties of these important molecules. The behavior of both single-stranded and double-stranded nucleic acids under changing solution conditions, such as ionic strength, pH and temperature, has been studied in detail. There has also been progress in using these techniques to study both the kinetics and equilibrium thermodynamics of DNA-protein interactions. These studies generate unique insights into the functions of these proteins in the cell.
AB - Experiments in which single molecules of RNA and DNA are stretched, and the resulting force as a function of extension is measured have yielded new information about the physical, chemical and biological properties of these important molecules. The behavior of both single-stranded and double-stranded nucleic acids under changing solution conditions, such as ionic strength, pH and temperature, has been studied in detail. There has also been progress in using these techniques to study both the kinetics and equilibrium thermodynamics of DNA-protein interactions. These studies generate unique insights into the functions of these proteins in the cell.
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U2 - 10.1016/S0959-440X(02)00340-8
DO - 10.1016/S0959-440X(02)00340-8
M3 - Review article
C2 - 12127451
AN - SCOPUS:0036600949
SN - 0959-440X
VL - 12
SP - 330
EP - 336
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 3
ER -