Free radical scavengers in mercuric chloride-induced acute renal failure in the rat

M. S. Paller

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Oxygen free radicals have recently been found to mediate cell injury after ischemia in the kidney. We sought to determine whether oxygen free radicals mediate damage in mercuric chloride (HgCl2)-induced acute renal failure, a toxic model of acute renal failure. Neither superoxide dismutase nor allopurinol, which scavenges or inhibits production of superoxide radical, respectively, provided protection against renal dysfunction after HgCl2. Similarly, the hydroxyl radical scavengers tryptophan, N-acetyl-tryptophan, and ascorbic acid were unable to protect against HgCl2. However, dimethylthiourea and dimethyl sulfoxide, both hydroxyl radical scavengers, were beneficial. Dimethylthiourea completely prevented the rise in plasma creatinine concentration after HgCl2. In control rats plasma creatinine concentration rose from 0.4 mg/dl to 3.2 ± 0.8, 5.1 ± 1.0, and 6.1 ± 1.6 mg/dl at 24, 48, and 72 hours after HgCl2. Dimethylthiourea-treated rats had plasma creatinine concentration <0.5 mg/dl at all times. Furthermore, a mixture of HgCl2 and equimolar amounts of dimethylthiouria was less toxic than HgCl2 alone. Dimethyl sulfoxide attenuated the HgCl2-induced rise in creatinine concentration: 1.3 ± 0.2, 3.2 ± 0.3, and 3.1 ± 0.2 mg/dl at 24, 48, and 72 hours after HgCl2. Measurement of kidney malondialdehyde content after HgCl2 provided no evidence for oxygen free radical-mediated lipid peroxidation. We conclude that there is no convincing role for oxygen free radicals in the pathogenesis of HgCl2-induced acute renal failure. The ability of dimethylthiourea and dimethyl sulfoxide to protect against HgCl2-induced renal dysfunction may be related to their ability to form complexes with Hg2+.

Original languageEnglish (US)
Pages (from-to)459-463
Number of pages5
JournalJournal of Laboratory and Clinical Medicine
Volume105
Issue number4
StatePublished - 1985

Fingerprint

Dive into the research topics of 'Free radical scavengers in mercuric chloride-induced acute renal failure in the rat'. Together they form a unique fingerprint.

Cite this