Generation of surface-bound multicomponent protein gradients

Kechun Zhang, Ayae Sugawara, David A. Tirrell

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


(Figure Presented) Spatial control of bioactive ligands is achieved by integrating microfluidics and protein engineering. The proteins of interest are mixed in a gradient generator and immobilized on artificial polypeptide scaffolds through the strong association of heterodimeric ZE/ZR leucine zipper pairs. Protein densities and gradient shapes are easily controlled and varied in this method.

Original languageEnglish (US)
Pages (from-to)2617-2619
Number of pages3
Issue number16
StatePublished - Nov 2 2009


  • Cell adhesion
  • Coiled coils
  • Microfluidics
  • Proteins
  • Surface functionalization

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