Glycoproteins and glycosylation: Apolipoprotein C3 glycoforms by top-down MALDI-TOF mass spectrometry

Yan Zhang, Alan R. Sinaiko, Gary L. Nelsestuen

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Scopus citations

Abstract

Apolipoprotein C3 (ApoC3) is synthesized in liver so that levels or isoform distributions may constitute indicators of liver pathogenesis. The glycoforms of intact protein ApoC3 in serum or plasma can be readily analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry after a one-step extraction using a C4 reverse-phase ZipTip. Glycoform distributions were sensitive to severe systemic diseases such as sepsis or liver diseases such as chronic hepatitis C and alcoholic liver cirrhosis. Glycoisoform distributions were also altered in persons with elevated body mass index and were corrected to normal distribution by metformin, a common drug used for diabetes therapy. This simple method may offer an approach to analysis of health and the mechanism of drug therapies.

Original languageEnglish (US)
Title of host publicationLiver Proteomics
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages141-150
Number of pages10
ISBN (Print)9781617799587
DOIs
StatePublished - Jan 1 2012

Publication series

NameMethods in Molecular Biology
Volume909
ISSN (Print)1064-3745

Keywords

  • Apolipoprotein C3
  • Glycoforms
  • Glycosylation
  • Liver diseases
  • MALDI-TOF
  • Mass spectrometry
  • Plasma biomarker
  • Protein profiling

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