High-resolution RH map of horse chromosome 22 reveals a putative ancestral vertebrate chromosome

Ashley Gustafson-Seabury; Terje Raudsepp; Glenda Goh; Srinivas R. Kata; Michelle L. Wagner; Teruaki Tozaki; James R. Mickelson; James E. Womack; Loren C. Skow; Bhanu P. Chowdhary

doi:10.1016/j.ygeno.2004.10.012

High-resolution RH map of horse chromosome 22 reveals a putative ancestral vertebrate chromosome

Ashley Gustafson-Seabury, Terje Raudsepp, Glenda Goh, Srinivas R. Kata, Michelle L. Wagner, Teruaki Tozaki, James R. Mickelson, James E. Womack, Loren C. Skow, Bhanu P. Chowdhary

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

High-resolution gene maps of individual equine chromosomes are essential to identify genes governing traits of economic importance in the horse. In pursuit of this goal we herein report the generation of a dense map of horse chromosome 22 (ECA22) comprising 83 markers, of which 52 represent specific genes and 31 are microsatellites. The map spans 831 cR over an estimated 64 Mb of physical length of the chromosome, thus providing markers at ∼770 kb or 10 cR intervals. Overall, the resolution of the map is to date the densest in the horse and is the highest for any of the domesticated animal species for which annotated sequence data are not yet available. Comparative analysis showed that ECA22 shares remarkable conservation of gene order along the entire length of dog chromosome 24, something not yet found for an autosome in evolutionarily diverged species. Comparison with human, mouse, and rat homologues shows that ECA22 can be traced as two conserved linkage blocks, each related to individual arms of the human homologue-HSA20. Extending the comparison to the chicken genome showed that one of the ECA22 blocks that corresponds to HSA20q shares synteny conservation with chicken chromosome 20, suggesting the segment to be ancestral in mammals and birds.

Original language	English (US)
Pages (from-to)	188-200
Number of pages	13
Journal	Genomics
Volume	85
Issue number	2
DOIs	https://doi.org/10.1016/j.ygeno.2004.10.012
State	Published - Feb 2005

Bibliographical note

Funding Information:
This project was funded by grants from the Texas Higher Education Board (ARP 010366-0162-2001, B.P.C.; ATP 000517-0306-2003, B.P.C., J.E.W.), NRICGP/USDA Grant 2003-03687 (B.P.C.), the Texas Equine Research Foundation (B.P.C., L.C.S.), the Link Endowment (B.P.C., L.C.S.), the American Quarter Horse Association, and the Dorothy Russell Havemeyer Foundation. Additional support was available from the USDA-NRSP-8 Coordinators Fund. We thank Dr. Pat Venta, Michigan State University, for design and supply of several primer pairs.

Keywords

Comparative map
ECA22
FISH
Gene mapping
Horse
Human
Mouse
RH map

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@article{f56e35cdeb0d42879380a45fa682d3c0,

title = "High-resolution RH map of horse chromosome 22 reveals a putative ancestral vertebrate chromosome",

abstract = "High-resolution gene maps of individual equine chromosomes are essential to identify genes governing traits of economic importance in the horse. In pursuit of this goal we herein report the generation of a dense map of horse chromosome 22 (ECA22) comprising 83 markers, of which 52 represent specific genes and 31 are microsatellites. The map spans 831 cR over an estimated 64 Mb of physical length of the chromosome, thus providing markers at ∼770 kb or 10 cR intervals. Overall, the resolution of the map is to date the densest in the horse and is the highest for any of the domesticated animal species for which annotated sequence data are not yet available. Comparative analysis showed that ECA22 shares remarkable conservation of gene order along the entire length of dog chromosome 24, something not yet found for an autosome in evolutionarily diverged species. Comparison with human, mouse, and rat homologues shows that ECA22 can be traced as two conserved linkage blocks, each related to individual arms of the human homologue-HSA20. Extending the comparison to the chicken genome showed that one of the ECA22 blocks that corresponds to HSA20q shares synteny conservation with chicken chromosome 20, suggesting the segment to be ancestral in mammals and birds.",

keywords = "Comparative map, ECA22, FISH, Gene mapping, Horse, Human, Mouse, RH map",

author = "Ashley Gustafson-Seabury and Terje Raudsepp and Glenda Goh and Kata, {Srinivas R.} and Wagner, {Michelle L.} and Teruaki Tozaki and Mickelson, {James R.} and Womack, {James E.} and Skow, {Loren C.} and Chowdhary, {Bhanu P.}",

note = "Funding Information: This project was funded by grants from the Texas Higher Education Board (ARP 010366-0162-2001, B.P.C.; ATP 000517-0306-2003, B.P.C., J.E.W.), NRICGP/USDA Grant 2003-03687 (B.P.C.), the Texas Equine Research Foundation (B.P.C., L.C.S.), the Link Endowment (B.P.C., L.C.S.), the American Quarter Horse Association, and the Dorothy Russell Havemeyer Foundation. Additional support was available from the USDA-NRSP-8 Coordinators Fund. We thank Dr. Pat Venta, Michigan State University, for design and supply of several primer pairs.",

year = "2005",

month = feb,

doi = "10.1016/j.ygeno.2004.10.012",

language = "English (US)",

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T1 - High-resolution RH map of horse chromosome 22 reveals a putative ancestral vertebrate chromosome

AU - Gustafson-Seabury, Ashley

AU - Raudsepp, Terje

AU - Goh, Glenda

AU - Kata, Srinivas R.

AU - Wagner, Michelle L.

AU - Tozaki, Teruaki

AU - Mickelson, James R.

AU - Womack, James E.

AU - Skow, Loren C.

AU - Chowdhary, Bhanu P.

N1 - Funding Information: This project was funded by grants from the Texas Higher Education Board (ARP 010366-0162-2001, B.P.C.; ATP 000517-0306-2003, B.P.C., J.E.W.), NRICGP/USDA Grant 2003-03687 (B.P.C.), the Texas Equine Research Foundation (B.P.C., L.C.S.), the Link Endowment (B.P.C., L.C.S.), the American Quarter Horse Association, and the Dorothy Russell Havemeyer Foundation. Additional support was available from the USDA-NRSP-8 Coordinators Fund. We thank Dr. Pat Venta, Michigan State University, for design and supply of several primer pairs.

PY - 2005/2

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N2 - High-resolution gene maps of individual equine chromosomes are essential to identify genes governing traits of economic importance in the horse. In pursuit of this goal we herein report the generation of a dense map of horse chromosome 22 (ECA22) comprising 83 markers, of which 52 represent specific genes and 31 are microsatellites. The map spans 831 cR over an estimated 64 Mb of physical length of the chromosome, thus providing markers at ∼770 kb or 10 cR intervals. Overall, the resolution of the map is to date the densest in the horse and is the highest for any of the domesticated animal species for which annotated sequence data are not yet available. Comparative analysis showed that ECA22 shares remarkable conservation of gene order along the entire length of dog chromosome 24, something not yet found for an autosome in evolutionarily diverged species. Comparison with human, mouse, and rat homologues shows that ECA22 can be traced as two conserved linkage blocks, each related to individual arms of the human homologue-HSA20. Extending the comparison to the chicken genome showed that one of the ECA22 blocks that corresponds to HSA20q shares synteny conservation with chicken chromosome 20, suggesting the segment to be ancestral in mammals and birds.

AB - High-resolution gene maps of individual equine chromosomes are essential to identify genes governing traits of economic importance in the horse. In pursuit of this goal we herein report the generation of a dense map of horse chromosome 22 (ECA22) comprising 83 markers, of which 52 represent specific genes and 31 are microsatellites. The map spans 831 cR over an estimated 64 Mb of physical length of the chromosome, thus providing markers at ∼770 kb or 10 cR intervals. Overall, the resolution of the map is to date the densest in the horse and is the highest for any of the domesticated animal species for which annotated sequence data are not yet available. Comparative analysis showed that ECA22 shares remarkable conservation of gene order along the entire length of dog chromosome 24, something not yet found for an autosome in evolutionarily diverged species. Comparison with human, mouse, and rat homologues shows that ECA22 can be traced as two conserved linkage blocks, each related to individual arms of the human homologue-HSA20. Extending the comparison to the chicken genome showed that one of the ECA22 blocks that corresponds to HSA20q shares synteny conservation with chicken chromosome 20, suggesting the segment to be ancestral in mammals and birds.

KW - Comparative map

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KW - FISH

KW - Gene mapping

KW - Horse

KW - Human

KW - Mouse

KW - RH map

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EP - 200

JO - Genomics

JF - Genomics

IS - 2

ER -

High-resolution RH map of horse chromosome 22 reveals a putative ancestral vertebrate chromosome

Abstract

Bibliographical note

Keywords

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