Highly sensitive fluorescent protein FRET detection using optofluidic lasers

Qiushu Chen; Xingwang Zhang; Yuze Sun; Michael Ritt; Sivaraj Sivaramakrishnan; Xudong Fan

doi:10.1039/c3lc50207d

Highly sensitive fluorescent protein FRET detection using optofluidic lasers

Qiushu Chen, Xingwang Zhang, Yuze Sun, Michael Ritt, Sivaraj Sivaramakrishnan, Xudong Fan

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

We achieved optofluidic protein lasing using genetically encoded fluorescent protein FRET pairs linked by length-tunable peptides. Up to 25-fold reduction in the donor laser emission was observed when the donor and the acceptor were brought to close proximity, as compared to only 17% reduction in the donor emission using the conventional FRET detection. Our work opens a door to a broad range of applications in studying protein-protein interactions and protein-drug interactions.

Original language	English (US)
Pages (from-to)	2679-2681
Number of pages	3
Journal	Lab on a chip
Volume	13
Issue number	14
DOIs	https://doi.org/10.1039/c3lc50207d
State	Published - Jul 21 2013

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abstract = "We achieved optofluidic protein lasing using genetically encoded fluorescent protein FRET pairs linked by length-tunable peptides. Up to 25-fold reduction in the donor laser emission was observed when the donor and the acceptor were brought to close proximity, as compared to only 17% reduction in the donor emission using the conventional FRET detection. Our work opens a door to a broad range of applications in studying protein-protein interactions and protein-drug interactions.",

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AU - Chen, Qiushu

AU - Zhang, Xingwang

AU - Sun, Yuze

AU - Ritt, Michael

AU - Sivaramakrishnan, Sivaraj

AU - Fan, Xudong

PY - 2013/7/21

Y1 - 2013/7/21

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AB - We achieved optofluidic protein lasing using genetically encoded fluorescent protein FRET pairs linked by length-tunable peptides. Up to 25-fold reduction in the donor laser emission was observed when the donor and the acceptor were brought to close proximity, as compared to only 17% reduction in the donor emission using the conventional FRET detection. Our work opens a door to a broad range of applications in studying protein-protein interactions and protein-drug interactions.

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Highly sensitive fluorescent protein FRET detection using optofluidic lasers

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