Homodimeric alkaline phosphatase located at Helicoverpa armigera Midgut, a putat ive receptor of Cry1Ac contains α-GaINAc in term inal glycan structure as interactive Epitope

Anindya Sarkar; Daniel Hess; Hossain A. Mondal; Santanu Banerjee; Hari C. Sharma; Sampa Das

doi:10.1021/pr8006528

Homodimeric alkaline phosphatase located at Helicoverpa armigera Midgut, a putat ive receptor of Cry1Ac contains α-GaINAc in term inal glycan structure as interactive Epitope

Anindya Sarkar, Daniel Hess, Hossain A. Mondal, Santanu Banerjee, Hari C. Sharma, Sampa Das

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Abstract

Helicoverpa armigera causes massive yield loss of various crops globally. Bacillus thuringiensis coded Cry1Ac toxin is effective against the pest. Through the present investigation, an alkaline phosphatase type putative receptor of the toxin was identified in the target insect gut BBMV. Lectin-ligand immunoblot assay detected the presence of α-GalNAc residue at the nonreducing terminal of the glycan structure in the membrane bound HaALP protein which mediates the toxin-receptor interaction.

Original language	English (US)
Pages (from-to)	1838-1848
Number of pages	11
Journal	Journal of Proteome Research
Volume	8
Issue number	4
DOIs	https://doi.org/10.1021/pr8006528
State	Published - Apr 3 2009
Externally published	Yes

Keywords

Alkaline phosphatase
Cry l Ac
Helicoverpa armigera
Putative receptor
Toxin receptor interaction

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Homodimeric alkaline phosphatase located at Helicoverpa armigera Midgut, a putat ive receptor of Cry1Ac contains α-GaINAc in term inal glycan structure as interactive Epitope. / Sarkar, Anindya; Hess, Daniel; Mondal, Hossain A. et al.
In: Journal of Proteome Research, Vol. 8, No. 4, 03.04.2009, p. 1838-1848.

Research output: Contribution to journal › Article › peer-review

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abstract = "Helicoverpa armigera causes massive yield loss of various crops globally. Bacillus thuringiensis coded Cry1Ac toxin is effective against the pest. Through the present investigation, an alkaline phosphatase type putative receptor of the toxin was identified in the target insect gut BBMV. Lectin-ligand immunoblot assay detected the presence of α-GalNAc residue at the nonreducing terminal of the glycan structure in the membrane bound HaALP protein which mediates the toxin-receptor interaction.",

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AU - Mondal, Hossain A.

AU - Banerjee, Santanu

AU - Sharma, Hari C.

AU - Das, Sampa

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AB - Helicoverpa armigera causes massive yield loss of various crops globally. Bacillus thuringiensis coded Cry1Ac toxin is effective against the pest. Through the present investigation, an alkaline phosphatase type putative receptor of the toxin was identified in the target insect gut BBMV. Lectin-ligand immunoblot assay detected the presence of α-GalNAc residue at the nonreducing terminal of the glycan structure in the membrane bound HaALP protein which mediates the toxin-receptor interaction.

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KW - Cry l Ac

KW - Helicoverpa armigera

KW - Putative receptor

KW - Toxin receptor interaction

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Homodimeric alkaline phosphatase located at Helicoverpa armigera Midgut, a putat ive receptor of Cry1Ac contains α-GaINAc in term inal glycan structure as interactive Epitope

Abstract

Keywords

UN SDGs

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