Hydrogen-Deuterium Exchange and Selective Labeling of Deprotonated Amino Acids and Peptides in the Gas Phase

Zhixin Tian, Lev Lis, Steven R. Kass

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Hydrogen-deuterium exchange reactions of deprotonated amino acids and small peptides were studied. Selective labeling can be carried out at the α-amino group of lysine (2 of 4 labile hydrogens undergo exchange with CF3CH2OD) and the guanidine side chain of arginine (3 of 6 labile hydrogens undergo exchange with CH3CH2OD and C6H5CH2OD). Differential labeling of peptides also was accomplished, and the extent of H/D exchange is dependent on the amino acids which are present as well as their order in the chain.

Original languageEnglish (US)
Pages (from-to)8-9
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number1
DOIs
StatePublished - Jan 9 2008

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