TY - JOUR
T1 - Identification of NK cell receptor ligands using a signaling reporter system.
AU - Iizuka, Yoshie Matsubayashi
AU - Somia, Nikunj V.
AU - Iizuka, Koho
PY - 2010
Y1 - 2010
N2 - NK cell responses are regulated by a balance of inhibitory and activating signals, reflecting the net effect of interactions between receptors and ligands on target and effector cell surfaces. The identification of ligands for orphan NK cell receptors is key to enhancing our understanding of NK cell biology. Here we describe a strategy (protocol) for the identification of ligands for orphan NK cell receptors using signaling reporter cells in combination with a virus rescue system.
AB - NK cell responses are regulated by a balance of inhibitory and activating signals, reflecting the net effect of interactions between receptors and ligands on target and effector cell surfaces. The identification of ligands for orphan NK cell receptors is key to enhancing our understanding of NK cell biology. Here we describe a strategy (protocol) for the identification of ligands for orphan NK cell receptors using signaling reporter cells in combination with a virus rescue system.
UR - http://www.scopus.com/inward/record.url?scp=77449152128&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77449152128&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-362-6_19
DO - 10.1007/978-1-60761-362-6_19
M3 - Article
C2 - 20033648
AN - SCOPUS:77449152128
SN - 1064-3745
VL - 612
SP - 285
EP - 297
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -