TY - JOUR
T1 - In vitro evolution of enzymes.
AU - Golynskiy, Misha V.
AU - Haugner, John C.
AU - Morelli, Aleardo
AU - Morrone, Dana
AU - Seelig, Burckhard
PY - 2013/8/30
Y1 - 2013/8/30
N2 - In the past decade, in vitro evolution techniques have been used to improve the performance or alter the activity of a number of different enzymes and have generated enzymes de novo. In this review, we provide an overview of the available in vitro methods, their application, and some general considerations for enzyme engineering in vitro. We discuss the advantages of in vitro over in vivo approaches and focus on ribosome display, mRNA display, DNA display technologies, and in vitro compartmentalization (IVC) methods. This review aims to help researchers determine which approach is best suited for their own experimental needs and to highlight that in vitro methods offer a promising route for enzyme engineering.
AB - In the past decade, in vitro evolution techniques have been used to improve the performance or alter the activity of a number of different enzymes and have generated enzymes de novo. In this review, we provide an overview of the available in vitro methods, their application, and some general considerations for enzyme engineering in vitro. We discuss the advantages of in vitro over in vivo approaches and focus on ribosome display, mRNA display, DNA display technologies, and in vitro compartmentalization (IVC) methods. This review aims to help researchers determine which approach is best suited for their own experimental needs and to highlight that in vitro methods offer a promising route for enzyme engineering.
UR - http://www.scopus.com/inward/record.url?scp=84882996788&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84882996788&partnerID=8YFLogxK
M3 - Article
C2 - 23423890
SN - 1064-3745
VL - 978
SP - 73
EP - 92
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -