TY - JOUR
T1 - In vivo examination of membrane protein localization and degradation with green fluorescent protein
AU - Hampton, Randolph Y.
AU - Koning, Ann
AU - Wright, Robin
AU - Rine, Jasper
PY - 1996/1/23
Y1 - 1996/1/23
N2 - To test the utility of green fluorescent protein (GFP) as an in vivo reporter protein when fused to a membrane domain, we made a fusion protein between yeast hydroxymethylglutaryl-CoA reductase and GFP. Fusion proteins displayed spatial localization and regulated degradation consistent with the native hydroxymethylglutaryl-CoA reductase proteins. Thus, GFP should be useful in the study of both membrane protein localization and protein degradation in vivo.
AB - To test the utility of green fluorescent protein (GFP) as an in vivo reporter protein when fused to a membrane domain, we made a fusion protein between yeast hydroxymethylglutaryl-CoA reductase and GFP. Fusion proteins displayed spatial localization and regulated degradation consistent with the native hydroxymethylglutaryl-CoA reductase proteins. Thus, GFP should be useful in the study of both membrane protein localization and protein degradation in vivo.
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U2 - 10.1073/pnas.93.2.828
DO - 10.1073/pnas.93.2.828
M3 - Article
C2 - 8570643
AN - SCOPUS:0030069686
SN - 0027-8424
VL - 93
SP - 828
EP - 833
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 2
ER -