In vivo 13C NMR spectroscopy has the unique capability to measure metabolic fluxes noninvasively in the brain. Quantitative measurements of metabolic fluxes require analysis of the 13C labeling time courses obtained experimentally with a metabolic model. The present work reviews the ingredients necessary for a dynamic metabolic modeling study, with particular emphasis on practical issues.
Bibliographical noteFunding Information:
We would like to thank Dee Koski for technical assistance. This work was supported in part by NIH grants P41-RR08079, R01-NS38672, the Keck Foundation and the MIND Institute. The General Clinical Research Center was supported by NIH grant M01-RR00400.
- C NMR spectroscopy
- Glutamatergic neurotransmission
- Metabolic modeling
- TCA cycle