In vivo 13C NMR spectroscopy and metabolic modeling in the brain: a practical perspective

Pierre Gilles Henry; Gregor Adriany; Dinesh Deelchand; Rolf Gruetter; Malgorzata Marjanska; Gülin Öz; Elizabeth R. Seaquist; Alexander Shestov; Kâmil Uǧurbil

doi:10.1016/j.mri.2006.01.003

In vivo ¹³C NMR spectroscopy and metabolic modeling in the brain: a practical perspective

Pierre Gilles Henry, Gregor Adriany, Dinesh Deelchand, Rolf Gruetter, Malgorzata Marjanska, Gülin Öz, Elizabeth R. Seaquist, Alexander Shestov, Kâmil Uǧurbil

Research output: Contribution to journal › Review article › peer-review

88 Scopus citations

Abstract

In vivo ¹³C NMR spectroscopy has the unique capability to measure metabolic fluxes noninvasively in the brain. Quantitative measurements of metabolic fluxes require analysis of the ¹³C labeling time courses obtained experimentally with a metabolic model. The present work reviews the ingredients necessary for a dynamic metabolic modeling study, with particular emphasis on practical issues.

Original language	English (US)
Pages (from-to)	527-539
Number of pages	13
Journal	Magnetic Resonance Imaging
Volume	24
Issue number	4
DOIs	https://doi.org/10.1016/j.mri.2006.01.003
State	Published - May 2006

Bibliographical note

Funding Information:
We would like to thank Dee Koski for technical assistance. This work was supported in part by NIH grants P41-RR08079, R01-NS38672, the Keck Foundation and the MIND Institute. The General Clinical Research Center was supported by NIH grant M01-RR00400.

Keywords

C NMR spectroscopy
Compartmentation
Glutamatergic neurotransmission
Metabolic modeling
TCA cycle

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10.1016/j.mri.2006.01.003

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Cite this

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title = "In vivo 13C NMR spectroscopy and metabolic modeling in the brain: a practical perspective",

abstract = "In vivo 13C NMR spectroscopy has the unique capability to measure metabolic fluxes noninvasively in the brain. Quantitative measurements of metabolic fluxes require analysis of the 13C labeling time courses obtained experimentally with a metabolic model. The present work reviews the ingredients necessary for a dynamic metabolic modeling study, with particular emphasis on practical issues.",

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AU - Adriany, Gregor

AU - Deelchand, Dinesh

AU - Gruetter, Rolf

AU - Marjanska, Malgorzata

AU - Öz, Gülin

AU - Seaquist, Elizabeth R.

AU - Shestov, Alexander

AU - Uǧurbil, Kâmil

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