Inability of real-time reverse transcriptase PCR assay to detect subtype H7 avian influenza viruses isolated from wild birds

Zheng Xing; Carol Cardona; Phuong Dao; Beate Crossley; Sharon Hietala; Walter Boyce

doi:10.1128/JCM.02426-07

Inability of real-time reverse transcriptase PCR assay to detect subtype H7 avian influenza viruses isolated from wild birds

Zheng Xing, Carol Cardona, Phuong Dao, Beate Crossley, Sharon Hietala, Walter Boyce

Host-Pathogen Interface Biology

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18 Scopus citations

Abstract

We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed for detecting H7 influenza viruses in poultry, is not reliable for wild bird surveillance.

Original language	English (US)
Pages (from-to)	1844-1846
Number of pages	3
Journal	Journal of clinical microbiology
Volume	46
Issue number	5
DOIs	https://doi.org/10.1128/JCM.02426-07
State	Published - May 2008

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title = "Inability of real-time reverse transcriptase PCR assay to detect subtype H7 avian influenza viruses isolated from wild birds",

abstract = "We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed for detecting H7 influenza viruses in poultry, is not reliable for wild bird surveillance.",

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AU - Hietala, Sharon

AU - Boyce, Walter

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Inability of real-time reverse transcriptase PCR assay to detect subtype H7 avian influenza viruses isolated from wild birds

Abstract

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