Influence of the Headgroup on the Interaction of Poly(ethylene oxide)-Poly(propylene oxide) Block Copolymers with Lipid Bilayers

Wenjia Zhang; Joseph M. Metzger; Benjamin J. Hackel; Frank S. Bates; Timothy P. Lodge

doi:10.1021/acs.jpcb.0c00553

Influence of the Headgroup on the Interaction of Poly(ethylene oxide)-Poly(propylene oxide) Block Copolymers with Lipid Bilayers

Wenjia Zhang, Joseph M. Metzger, Benjamin J. Hackel, Frank S. Bates, Timothy P. Lodge

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The lipid headgroup plays an important role in the association of polymers with lipid bilayer membranes. Herein, we report how a glycerol headgroup versus a choline headgroup affects the interaction of poly(ethylene oxide)-b-poly(propylene oxide) (PEO-PPO) block copolymers with lipid bilayer vesicles. Unilamellar vesicles composed of phosphatidylcholine and phosphatidylglycerol at various molar ratios were used as model membranes. The interactions between the block copolymers and lipid bilayers were quantified by pulsed-field gradient nuclear magnetic resonance (PFG-NMR) based on the distinctly different mobilities of free and bound polymers. All the investigated polymer species showed significantly higher binding with 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) sodium salt (POPG) liposomes than with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes, indicating stronger association with the glycerol headgroup compared to the choline headgroup. This effect did not become significant until the composition of mixed POPC/POPG liposomes contained more than 20 mol % POPG. A plausible explanation for the enhanced polymer binding with POPG invokes the role of hydrogen bonding between the glycerol headgroup and the ether moieties of the polymers.

Original language	English (US)
Pages (from-to)	2417-2424
Number of pages	8
Journal	Journal of Physical Chemistry B
Volume	124
Issue number	12
DOIs	https://doi.org/10.1021/acs.jpcb.0c00553
State	Published - Mar 26 2020

Bibliographical note

Funding Information:
This study was funded by the National Institutes of Health (grants HL122323; AR071349). We acknowledge Karen J. Haman for synthesizing the diblock polymers and Mihee Kim for useful discussions. We also acknowledge Letitia J. Yao for helping with the NMR experiments. NMR instrumentation was supported by the Office of the Vice President of Research, College of Science and Engineering, and the Department of Chemistry at the University of Minnesota. The Raman measurements were conducted in the Characterization Facility, University of Minnesota, with help from Bing Luo.

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural

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Influence of the Headgroup on the Interaction of Poly(ethylene oxide)-Poly(propylene oxide) Block Copolymers with Lipid Bilayers. / Zhang, Wenjia; Metzger, Joseph M.; Hackel, Benjamin J.; Bates, Frank S.; Lodge, Timothy P.

In: Journal of Physical Chemistry B, Vol. 124, No. 12, 26.03.2020, p. 2417-2424.

Research output: Contribution to journal › Article › peer-review

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title = "Influence of the Headgroup on the Interaction of Poly(ethylene oxide)-Poly(propylene oxide) Block Copolymers with Lipid Bilayers",

abstract = "The lipid headgroup plays an important role in the association of polymers with lipid bilayer membranes. Herein, we report how a glycerol headgroup versus a choline headgroup affects the interaction of poly(ethylene oxide)-b-poly(propylene oxide) (PEO-PPO) block copolymers with lipid bilayer vesicles. Unilamellar vesicles composed of phosphatidylcholine and phosphatidylglycerol at various molar ratios were used as model membranes. The interactions between the block copolymers and lipid bilayers were quantified by pulsed-field gradient nuclear magnetic resonance (PFG-NMR) based on the distinctly different mobilities of free and bound polymers. All the investigated polymer species showed significantly higher binding with 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) sodium salt (POPG) liposomes than with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes, indicating stronger association with the glycerol headgroup compared to the choline headgroup. This effect did not become significant until the composition of mixed POPC/POPG liposomes contained more than 20 mol % POPG. A plausible explanation for the enhanced polymer binding with POPG invokes the role of hydrogen bonding between the glycerol headgroup and the ether moieties of the polymers.",

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note = "Funding Information: This study was funded by the National Institutes of Health (grants HL122323; AR071349). We acknowledge Karen J. Haman for synthesizing the diblock polymers and Mihee Kim for useful discussions. We also acknowledge Letitia J. Yao for helping with the NMR experiments. NMR instrumentation was supported by the Office of the Vice President of Research, College of Science and Engineering, and the Department of Chemistry at the University of Minnesota. The Raman measurements were conducted in the Characterization Facility, University of Minnesota, with help from Bing Luo.",

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