Inheritance and bulked segregant analysis of leaf rust and stem rust resistance in durum wheat genotypes

Meriem Aoun, James A. Kolmer, Matthew N. Rouse, Shiaoman Chao, Worku Denbel Bulbula, Elias M. Elias, Maricelis Acevedo

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Leaf rust, caused by Puccinia triticina, and stem rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to P. triticina race BBBQJ and stem rust resistance (Sr) genes to P. graminis f. sp. tritici race TTKSK in durum accessions. Eight leaf-rustresistant genotypes were used to develop biparental populations. Accessions PI 192051 and PI 534304 were also resistant to P. graminis f. sp. tritici race TTKSK. The resulting progenies were phenotyped for leaf rust and stem rust response at seedling stage. The Lr and Sr genes were mapped in five populations using single-nucleotide polymorphisms and bulked segregant analysis. Five leaf-rust-resistant genotypes carried single dominant Lr genes whereas, in the remaining accessions, there was deviation from the expected segregation ratio of a single dominant Lr gene. Seven genotypes carried Lr genes different from those previously characterized in durum. The single dominant Lr genes in PI 209274, PI 244061, PI387263, and PI 313096 were mapped to chromosome arms 6BS, 2BS, 6BL, and 6BS, respectively. The Sr gene in PI 534304 mapped to 6AL and is most likely Sr13, while the Sr gene in PI 192051 could be uncharacterized in durum.

Original languageEnglish (US)
Pages (from-to)1496-1506
Number of pages11
JournalPhytopathology
Volume107
Issue number12
DOIs
StatePublished - Dec 2017

Bibliographical note

Funding Information:
We thank J. Huerta-Espino for kindly providing some of the P. triticina races used in this study; and A. Swank, J. Glasgow, and the wheat personnel at the EIAR-Debre Zeit Research Center for their technical support. This work was supported by the North Dakota Wheat Commission, the North Dakota State Board of Research and Education, and the Bill & Melinda Gates Foundation (OPP1133199).

Publisher Copyright:
© 2017 The American Phytopathological Society.

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