Inhibition of FGF signaling causes expansion of the endoderm in Xenopus

Sang Wook Cha, Yoo Seok Hwang, Jung Pil Chae, Sung Young Lee, Hyun Shik Lee, Ira Daar, Mae Ja Park, Jaebong Kim

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Fibroblast growth factor (FGF) is established as an initiator of signaling events critical for neurogenesis and mesoderm formation during early Xenopus embryogenesis. However, less is known about the role FGF signaling plays in endoderm specification. Here, we show for the first time that endoderm-specific genes are induced when FGF signaling is blocked in animal cap explants. This block of FGF signaling is also responsible for a significant enhancement of endodermal gene expression in animal cap explants that are injected with a dominant-negative BMP-4 receptor (DNBR) RNA or treated with activin, however, neural and mesoderm gene expression is diminished. Consistent with these results, the injection of dominant-negative FGF receptor (DNFR) RNA expands endodermal cell fate boundaries while FGF treatment dramatically reduces endoderm in whole embryos. Taken together, these results indicate that inhibition of FGF signaling promotes endoderm formation, whereas the presence of active FGF signaling is necessary for neurogenesis/mesoderm formation.

Original languageEnglish (US)
Pages (from-to)100-106
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Feb 27 2004

Bibliographical note

Funding Information:
Grant No. 01-PJ1-PG3-20700-0040: Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea.

Copyright 2017 Elsevier B.V., All rights reserved.


  • Embryogenesis
  • FGF
  • Mesendoderm specification
  • Xenopus

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