Instrumentation for chemical cytometry

Sergey N. Krylov, Dieter A. Starke, Edgar A. Arriaga, Zheru Zhang, Nora W.C. Chan, Monica M. Palcic, Norman J. Dovichi

Research output: Contribution to journalArticlepeer-review

130 Scopus citations

Abstract

Capillary electrophoresis is ideally suited to chemical analysis of individual cells. Small mammalian somatic cells (~15 pm in diameter) can be analyzed by injecting the intact cell into a capillary, lysing the cell, separating and detecting the cellular components, and reconditioning the capillary prior to the next injection. In this paper, we report on technical improvements to single-cell analysis. We designed an inexpensive multipurpose single-cell injector that facilitates the following: (i) monitoring of injection, (ii) reproducible pressure- or electrokinetic-driven injection of the cell, (iii) complete cell lysis by SDS within 30 s of injection, and (iv) pressure-driven capillary reconditioning. Furthermore, we report on the analysis of glycosylation and glycolysis in single human carcinoma cells (HT29 cell line). The reliability and quality of the analysis is confirmed by comparing electropherograms from single cells and those from purified cell extracts.

Original languageEnglish (US)
Pages (from-to)872-877
Number of pages6
JournalAnalytical Chemistry
Volume72
Issue number4
DOIs
StatePublished - Feb 15 2000

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