Abstract
Previous studies showed that crosslinking of IgE-FcεRI complexes on RBL-2H3 mast cells causes their association with isolated detergent-resistant membranes, also known as lipid rafts, in a cholesterol-dependent process that precedes initiation of signaling by these receptors. To investigate these interactions on intact cells, we examined the co-redistribution of raft components with crosslinked IgE-FcεRI using confocal microscopy. After several hours of crosslinking at 4°C, the glycosylphosphatidylinositol-linked protein Thy-1 and the Src-family tyrosine kinase Lyn co-redistribute with IgE-FcεRI in large patches at the plasma membrane. Under these conditions, F-actin also undergoes dramatic co-segregation with FcεRI and raft components but is dispersed following a brief warm-up to 37°C. When crosslinking of IgE-FcεRI is initiated at higher temperatures, co-redistribution of raft components with patched FcεRI is not readily detected unless stimulated F-actin polymerization is inhibited by cytochalasin D. In parallel, cytochalasin D converts transient antigen-stimulated tyrosine phosphorylation to a more sustained response. Sucrose gradient analysis of lysed cells reveals that crosslinked IgE-FcεRI remains associated with lipid rafts throughout the time course of the transient phosphorylation response but undergoes a time-dependent shift to higher density that is prevented by cytochalasin D. Our results indicate that interactions between Lyn and crosslinked IgE-FcεRI are regulated by stimulated F-actin polymerization, and this is best explained by a segregation of anchored raft components from more mobile ones.
Original language | English (US) |
---|---|
Pages (from-to) | 1009-1019 |
Number of pages | 11 |
Journal | Journal of cell science |
Volume | 113 |
Issue number | 6 |
State | Published - 2000 |
Keywords
- Detergent-resistant membrane
- IgE receptor
- Lyn kinase
- Mast cell
- Membrane-cytoskeletal interaction