IRX3/5 regulate mitotic chromatid segregation and limb bud shape

Hirotaka Tao; Jean Philippe Lambert; Theodora M. Yung; Min Zhu; Noah A. Hahn; Danyi Li; Kimberly Lau; Kendra Sturgeon; Vijitha Puviindran; Xiaoyun Zhang; Wuming Gong; Xiao Xiao Chen; Gregory Anderson; Daniel J. Garry; R. Mark Henkelman; Yu Sun; Angelo Iulianella; Yasuhiko Kawakami; Anne Claude Gingras; Chi Chung Hui; Sevan Hopyan

doi:10.1242/dev.180042

IRX3/5 regulate mitotic chromatid segregation and limb bud shape

Hirotaka Tao, Jean Philippe Lambert, Theodora M. Yung, Min Zhu, Noah A. Hahn, Danyi Li, Kimberly Lau, Kendra Sturgeon, Vijitha Puviindran, Xiaoyun Zhang, Wuming Gong, Xiao Xiao Chen, Gregory Anderson, Daniel J. Garry, R. Mark Henkelman, Yu Sun, Angelo Iulianella, Yasuhiko Kawakami, Anne Claude Gingras, Chi Chung HuiSevan Hopyan

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Pattern formation is influenced by transcriptional regulation as well as by morphogenetic mechanisms that shape organ primordia, although factors that link these processes remain under-appreciated. Here we show that, apart from their established transcriptional roles in pattern formation, IRX3/5 help to shape the limb bud primordium by promoting the separation and intercalation of dividing mesodermal cells. Surprisingly, IRX3/5 are required for appropriate cell cycle progression and chromatid segregation during mitosis, possibly in a nontranscriptional manner. IRX3/5 associate with, promote the abundance of, and share overlapping functions with co-regulators of cell division such as the cohesin subunits SMC1, SMC3, NIPBL and CUX1. The findings imply that IRX3/5 coordinate early limb bud morphogenesis with skeletal pattern formation.

Original language	English (US)
Journal	Development (Cambridge, England)
Volume	147
Issue number	19
DOIs	https://doi.org/10.1242/dev.180042
State	Published - Oct 5 2020

Bibliographical note

Publisher Copyright:
© 2020. Published by The Company of Biologists Ltd.

Keywords

Cohesin
Iroquois
Limb development
Mesodermal cell intercalations
Mitotic chromatid segregation
Morphogenesis

Access

10.1242/dev.180042

OpenUrl availability

Full text

Cite this

Tao, H., Lambert, J. P., Yung, T. M., Zhu, M., Hahn, N. A., Li, D., Lau, K., Sturgeon, K., Puviindran, V., Zhang, X., Gong, W., Chen, X. X., Anderson, G., Garry, D. J., Henkelman, R. M., Sun, Y., Iulianella, A., Kawakami, Y., Gingras, A. C., ... Hopyan, S. (2020). IRX3/5 regulate mitotic chromatid segregation and limb bud shape. Development (Cambridge, England), 147(19). https://doi.org/10.1242/dev.180042

Tao, H, Lambert, JP, Yung, TM, Zhu, M, Hahn, NA, Li, D, Lau, K, Sturgeon, K, Puviindran, V, Zhang, X, Gong, W, Chen, XX, Anderson, G, Garry, DJ, Henkelman, RM, Sun, Y, Iulianella, A, Kawakami, Y, Gingras, AC, Hui, CC & Hopyan, S 2020, 'IRX3/5 regulate mitotic chromatid segregation and limb bud shape', Development (Cambridge, England), vol. 147, no. 19. https://doi.org/10.1242/dev.180042

@article{8287f20eace4489798e9f642a1551c10,

title = "IRX3/5 regulate mitotic chromatid segregation and limb bud shape",

abstract = "Pattern formation is influenced by transcriptional regulation as well as by morphogenetic mechanisms that shape organ primordia, although factors that link these processes remain under-appreciated. Here we show that, apart from their established transcriptional roles in pattern formation, IRX3/5 help to shape the limb bud primordium by promoting the separation and intercalation of dividing mesodermal cells. Surprisingly, IRX3/5 are required for appropriate cell cycle progression and chromatid segregation during mitosis, possibly in a nontranscriptional manner. IRX3/5 associate with, promote the abundance of, and share overlapping functions with co-regulators of cell division such as the cohesin subunits SMC1, SMC3, NIPBL and CUX1. The findings imply that IRX3/5 coordinate early limb bud morphogenesis with skeletal pattern formation.",

keywords = "Cohesin, Iroquois, Limb development, Mesodermal cell intercalations, Mitotic chromatid segregation, Morphogenesis",

author = "Hirotaka Tao and Lambert, {Jean Philippe} and Yung, {Theodora M.} and Min Zhu and Hahn, {Noah A.} and Danyi Li and Kimberly Lau and Kendra Sturgeon and Vijitha Puviindran and Xiaoyun Zhang and Wuming Gong and Chen, {Xiao Xiao} and Gregory Anderson and Garry, {Daniel J.} and Henkelman, {R. Mark} and Yu Sun and Angelo Iulianella and Yasuhiko Kawakami and Gingras, {Anne Claude} and Hui, {Chi Chung} and Sevan Hopyan",

note = "Publisher Copyright: {\textcopyright} 2020. Published by The Company of Biologists Ltd.",

year = "2020",

month = oct,

day = "5",

doi = "10.1242/dev.180042",

language = "English (US)",

volume = "147",

journal = "Development (Cambridge, England)",

issn = "0950-1991",

publisher = "Company of Biologists Ltd",

number = "19",

}

TY - JOUR

T1 - IRX3/5 regulate mitotic chromatid segregation and limb bud shape

AU - Tao, Hirotaka

AU - Lambert, Jean Philippe

AU - Yung, Theodora M.

AU - Zhu, Min

AU - Hahn, Noah A.

AU - Li, Danyi

AU - Lau, Kimberly

AU - Sturgeon, Kendra

AU - Puviindran, Vijitha

AU - Zhang, Xiaoyun

AU - Gong, Wuming

AU - Chen, Xiao Xiao

AU - Anderson, Gregory

AU - Garry, Daniel J.

AU - Henkelman, R. Mark

AU - Sun, Yu

AU - Iulianella, Angelo

AU - Kawakami, Yasuhiko

AU - Gingras, Anne Claude

AU - Hui, Chi Chung

AU - Hopyan, Sevan

PY - 2020/10/5

Y1 - 2020/10/5

N2 - Pattern formation is influenced by transcriptional regulation as well as by morphogenetic mechanisms that shape organ primordia, although factors that link these processes remain under-appreciated. Here we show that, apart from their established transcriptional roles in pattern formation, IRX3/5 help to shape the limb bud primordium by promoting the separation and intercalation of dividing mesodermal cells. Surprisingly, IRX3/5 are required for appropriate cell cycle progression and chromatid segregation during mitosis, possibly in a nontranscriptional manner. IRX3/5 associate with, promote the abundance of, and share overlapping functions with co-regulators of cell division such as the cohesin subunits SMC1, SMC3, NIPBL and CUX1. The findings imply that IRX3/5 coordinate early limb bud morphogenesis with skeletal pattern formation.

AB - Pattern formation is influenced by transcriptional regulation as well as by morphogenetic mechanisms that shape organ primordia, although factors that link these processes remain under-appreciated. Here we show that, apart from their established transcriptional roles in pattern formation, IRX3/5 help to shape the limb bud primordium by promoting the separation and intercalation of dividing mesodermal cells. Surprisingly, IRX3/5 are required for appropriate cell cycle progression and chromatid segregation during mitosis, possibly in a nontranscriptional manner. IRX3/5 associate with, promote the abundance of, and share overlapping functions with co-regulators of cell division such as the cohesin subunits SMC1, SMC3, NIPBL and CUX1. The findings imply that IRX3/5 coordinate early limb bud morphogenesis with skeletal pattern formation.

KW - Cohesin

KW - Iroquois

KW - Limb development

KW - Mesodermal cell intercalations

KW - Mitotic chromatid segregation

KW - Morphogenesis

UR - http://www.scopus.com/inward/record.url?scp=85092681940&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85092681940&partnerID=8YFLogxK

U2 - 10.1242/dev.180042

DO - 10.1242/dev.180042

M3 - Article

C2 - 32907847

AN - SCOPUS:85092681940

SN - 0950-1991

VL - 147

JO - Development (Cambridge, England)

JF - Development (Cambridge, England)

IS - 19

ER -

IRX3/5 regulate mitotic chromatid segregation and limb bud shape

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this