Isolation of a Bradyrhizobium japonicum serogroup 123 mutant which has an extended host range for nodulation-restricting soybean genotypes

A. K. Judd, M. J. Sadowsky, A. A. Bhagwat, P. B. Cregan, R. L. Liu

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Abstract

We have isolated a Bradyrhizobium japonicum USDA 438 (serogroup 123) mutant which has the ability to form nodules on serogroup 123 nodulation-restricting plant introduction genotypes soybeans the Rj4 allele. The identity of the mutant was confirmed by using a serocluster 123-specific DNA probe, restriction fragment length polymorphism analysis, and serogroup-specific fluorescent antibodies. While the mutant contains Tn5 inserted into a cryptic, non nod gene-containing locus, site-directed mutagenesis and complementation studies indicated that the transposon is not responsible for host-range extension. The mutant and the wild-type parent had the same chromatographic profiles of [14C]acetate-labelled extracellular B. japonicum.

Original languageEnglish (US)
Pages (from-to)205-209
Number of pages5
JournalFEMS Microbiology Letters
Volume106
Issue number2
DOIs
StatePublished - Feb 5 1993

Keywords

  • Bradyrhizobium japonicum
  • Host range-extension
  • Nodulation
  • Nodulation-restriction
  • Serogroup 123
  • Soybean plant introduction genotypes

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