Lessons learned: Rearing the crown-boring weevil, ceutorhynchus scrobicollis (coleoptera: Curculionidae), in containment for biological control of garlic mustard (alliaria petiolata)

Elizabeth J. Katovich, Roger L. Becker, Esther Gerber, Hariet L. Hinz, Ghislaine Cortat

Research output: Contribution to journalArticlepeer-review

Abstract

In this paper, we describe lessons learned and protocols developed after a decade of rearing Ceutorhynchus scrobicollis Nerenscheimer and Wagner (Coleoptera: Curculi-onidae) in a Biosafety Level 2 containment facility. We have developed these protocols in anticipation of approval to release C. scrobicollis in North America for the biocontrol of garlic mustard. The rearing protocol tried to minimize the potential of attack by the adult parasitoid, Perilitus consuetor Nees (Hymenoptera: Braconidae), which may be present in field collected C. scrobicollis from Europe to prevent inadvertent introduction of parasitoids into North America. All C. scrobicollis used for our quarantine rearing were field collected near Berlin, Germany. We have successfully reared C. scrobicollis on caged garlic mustard plants in a growth chamber by alternating temperatures and photoperiods to simulate those in its native range. In Germany, C. scrobicollis produces one generation per year and F1 adults emerge in late May. In containment, a new generation of adults emerged an average of 108 days after adults were placed on plants. We found the optimal time spent to collect F1 adults was four weeks after the appearance of the first F1 adult, with 95% of potential adults collected. Simulating a three-month summer aestivation period, followed by a week of fall, and three weeks of winter conditions resulted in optimum levels of oviposition in F1 females. Larvae first hatched 8-to-10 days after adults were placed on plants at 15/14 C day/night temperatures with a 9.5 hour photoperiod. We therefore recommend that C. scrobicollis adults are removed from garlic mustard rosettes after 8 days. This will maximize the period of female oviposition while minimizing the time when larvae are available for attack from P. consuetor.

Original languageEnglish (US)
Article number6
Pages (from-to)78-93
Number of pages16
JournalGreat Lakes Entomologist
Volume52
Issue number3-4
StatePublished - Sep 1 2019

Bibliographical note

Funding Information:
The authors would like to acknowledge: Richard C. Reardon, USDA Forest Service, Forest Health Technology Enterprise Team; USDA Forest Service; Minnesota Department of Natural Resources; University of Minnesota Invasive Terrestrial Plant Pest Center; and the Minnesota Environmental and Natural Resources Trust Fund as recommended by the Legislative Citizen Commission on Minnesota Resources for funding this work. CABI is an international intergovernmental organisation, and we gratefully acknowledge the core financial support from our member countries (and lead agencies) including the United Kingdom (Department for International Development), China (Chinese Ministry of Agriculture), Australia (Australian Centre for International Agricultural Research), Canada (Agriculture and Agri-Food Canada), Netherlands (Directorate-General for International Cooperation) and Switzerland (Swiss Agency for Development and Cooperation). See http://www.cabi.org/about-cabi/who-we-work-with/key-donors/ for full details.

Funding Information:
The authors would like to acknowl edge: Richard C. Reardon, USDA Forest Service, Forest Health Technology Enterprise Team; USDA Forest Service; Minnesota Department of Natural Resources; University of Minnesota Invasive Terrestrial Plant Pest Center; and the Minnesota Environmental and Natural Resources Trust Fund as recommended by the Legislative Citizen Commission on Minnesota Resources for funding this work. CABI is an international intergovernmental organisation, and we gratefully acknowledge the core financial support from our member countries (and lead agencies) including the United Kingdom (Department for International Development), China (Chinese Ministry of Agriculture), Australia (Australian Centre for International Agricultural Research), Canada (Agriculture and Agri-Food Canada), Netherlands (Directorate-General for International Cooperation) and Switzerland (Swiss Agency for Development and Cooperation). See http:// www.cabi.org/about-cabi/who-we-work-with/ key-donors/ for full details.

Keywords

  • Alliaria petiolata
  • Biological control of weeds
  • Ceutorhynchus scrobicollis
  • Garlic mustard

Fingerprint Dive into the research topics of 'Lessons learned: Rearing the crown-boring weevil, ceutorhynchus scrobicollis (coleoptera: Curculionidae), in containment for biological control of garlic mustard (alliaria petiolata)'. Together they form a unique fingerprint.

Cite this