LIBS-based detection of antioxidant elements: A new strategy

Geeta Watal, Bechan Sharma, Prashant Kumar Rai, Dolly Jaiswal, Devendra K. Rai, Nilesh K. Rai, A. K. Rai

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

The present study deals with the scientific evaluation of antioxidant potential of aqueous extract of Trichosanthes dioica fruits on diabetes-induced oxidative stress of diabetic rats. The most effective dose of mg/kg bw of fruit aqueous extract was given orally to diabetic rats for 30 days. Different oxidative stress parameters were analyzed in various tissues of control and treated diabetic rats. The observed elevated level of lipid peroxidation (LPO) comes down significantly (p < 0.05) and decreased activities of antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) got increased (p < 0.05) significantly of diabetic rats on extract treatment. Laser-Induced Breakdown Spectroscopy (LIBS) has been used as an analytical tool to detect major and minor elements like Mg, Fe, Na, K, Zn, Ca, H, O, C, and N present in the extract. The higher concentration of Ca2+, Mg2+, and Fe2+, as reflected by their intensities are responsible for antioxidant potential of T. dioica.

Original languageEnglish (US)
Title of host publicationAdvanced Protocols in Oxidative Stress II
PublisherHumana Press Inc.
Pages275-285
Number of pages11
ISBN (Print)9781607614104
DOIs
StatePublished - 2010

Publication series

NameMethods in Molecular Biology
Volume594
ISSN (Print)1064-3745

Keywords

  • Diabetes
  • Indian herbs
  • LIBS
  • Trichosanthes dioica

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